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Functionalized Luminescent Oxide Nanoparticles as Biological Probes

Published online by Cambridge University Press:  01 February 2011

Domitille Giaume ,
Didier Casanova ,
Khalid Lahlil ,
Martin-Pierre Sauviat ,
Antigoni Alexandrou ,
Thierry Gacoin  and
Jean-Pierre Boilot
Domitille Giaume
Affiliation:
[email protected], Ecole Polytechnique, Laboratory of Condensed Matter Physics, route de Saclay, Palaiseau, 91128, France, 33 1 69 33 47 44, 33 1 69 33 30 04
Didier Casanova
Affiliation:
[email protected], Ecole Polytechnique, CNRS UMR 7645, INSERM U45L, Laboratory for Optics and Biosciences, route de Saclay, Palaiseau, N/A, 91128, France
Khalid Lahlil
Affiliation:
[email protected], Ecole Polytechnique, Laboratory of Condensed Matter Physics, route de Saclay, Palaiseau, N/A, 91128, France
Martin-Pierre Sauviat
Affiliation:
[email protected], Ecole Polytechnique, CNRS UMR 7645, INSERM U45L, Laboratory for Optics and Biosciences, route de Saclay, Palaiseau, N/A, 91128, France
Antigoni Alexandrou
Affiliation:
[email protected], Ecole Polytechnique, CNRS UMR 7645, INSERM U45L, Laboratory for Optics and Biosciences, route de Saclay, Palaiseau, N/A, 91128, France
Thierry Gacoin
Affiliation:
[email protected], Ecole Polytechnique, Laboratory of Condensed Matter Physics, route de Saclay, Palaiseau, N/A, 91128, France
Jean-Pierre Boilot
Affiliation:
[email protected], Ecole Polytechnique, Laboratory of Condensed Matter Physics, route de Saclay, Palaiseau, N/A, 91128, France
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Abstract

Synthesis and functionalization of nanoparticles of yttrium vanadate doped with europium is described. Synthesis of yttrium vanadate leads to ovoid nanoparticles, with an average size of 24 nm. These nanoparticles are unstable in water, and surface charge can be brought by silicate, as proven by PCS, Zetametry and IR analyses. The nanoparticle surface is then functionalized with glycidoxypropyltrimethoxysilane. The rafting is characterized by 29Si MAS NMR, and ThermoGravimetric Analyses. This alkoxysilane presents an epoxy function which can then react with a nucleophilic molecule. The functionalization is studied by 29Si MAS NMR and TGA, whereas opening of the epoxy ring is studied by 13C MAS NMR. As an example of further reactivity, we chose to graft on the nanoparticles the active part of saxitoxin, a guanidinium group. This last functionalization is proven by optical experiments performed on a cell in biological conditions.

Keywords

nanostructurecolloidluminescence
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 942: Symposium W – Colloidal Materials – Synthesis, Structure, and Applications , 2006 , 0942-W12-05
Copyright
Copyright © Materials Research Society 2006

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