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Freezing Assisted Protein Delivery by Using Polymeric Cryoprotectant

Published online by Cambridge University Press:  14 January 2014

Sana AHMED  and
Kazuaki MATSUMURA
Sana AHMED
Affiliation:
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292 Japan M.Tech (Chemical Synthesis and Process Technologies), Department of Chemistry, University of Delhi, Delhi-110007, India
Kazuaki MATSUMURA
Affiliation:
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292 Japan
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Abstract

A number of drug carrier systems such as liposomes, polymeric-nanoparticles, microparticles, polymeric micelles have been investigated for intracellular delivery. Among these liposomes are the potential drug vehicles for efficient cytosolic delivery. They have an adhesive property for cell membrane to encapsulate the drug or protein effectively and showing the enhanced absorption rate. One of the problems could be the difficulty of incorporation of the drug or protein into cell. Therefore many studies of the drug carriers have been developed to enhance the intracellular delivery of materials. Here we propose the novel method to improve the intracellular uptaking by using freeze concentration. Solutes are excluded from ice crystallization and concentrated in the remaining solution during freezing by freezing concentration. We have reported that polymeric cryoprotectant which is carboxylated poly-L-lysine was adsorbed on to the cell membrane during freezing and caused effective freeze concentration. In this study we investigated that delivery of protein effectively taking place by liposome as a carrier agent. It was successfully delivered protein to L929 cells via freeze concentration using polymeric cryoprotectant as a novel drug delivery.

Keywords

biomaterialmacromolecular structurebiomedical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1622: Symposium E – Fundamentals of Gels and Self-Assembled Polymer Systems , 2014 , pp. 123 - 127
Copyright
Copyright © Materials Research Society 2014 

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