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Enhancement of Antibody Binding on SiO2 Love Wave Sensor Surface using (3-glycidoxypropyl)trimethoxysilane

Published online by Cambridge University Press:  01 February 2011

Nicolas Moll ,
Duy Haï Dinh ,
Emilie Pascal ,
Corinne Déjous ,
Jean-Paul Pillot ,
Bernard Bennetau ,
Dominique Rebière ,
Daniel Moynet ,
Yan Mas  and
Jacques Pistré
Nicolas Moll
Affiliation:
[email protected], Laboratoire IXL, Equipe capteur, 351, cours de la Libération, Talence, N/A, 33405, France, +33(0)5 40 00 65 40, +33(0)5 56 37 15 45
Duy Haï Dinh
Affiliation:
[email protected], Laboratoire de Chimie Organique et Organométallique, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Emilie Pascal
Affiliation:
[email protected], Laboratoire d'Immunologie et Parasitologie, Université Bordeaux 2 Victor Segalen, 146, rue Léo Saignat, Bordeaux, N/A, 33076, France
Corinne Déjous
Affiliation:
[email protected], Laboratoire IXL, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Jean-Paul Pillot
Affiliation:
[email protected], Laboratoire de Chimie Organique et Organométallique, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Bernard Bennetau
Affiliation:
[email protected], Laboratoire de Chimie Organique et Organométallique, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Dominique Rebière
Affiliation:
[email protected], Laboratoire IXL, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
Daniel Moynet
Affiliation:
[email protected], Laboratoire d'Immunologie et Parasitologie, Université Bordeaux 2 Victor Segalen, 146, rue Léo Saignat, Bordeaux, N/A, 33076, France
Yan Mas
Affiliation:
[email protected], AGUR, 5, rue de la Feuillée, Bayonne, N/A, 64100, France
Jacques Pistré
Affiliation:
[email protected], Laboratoire IXL, 351, cours de la Libération, Université Bordeaux 1, Talence, N/A, 33405, France
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Abstract

An acoustic Love wave immunosensor dedicated to detect biological species such as bacteria, viruses or toxins is described in this paper. We present and analyze results of antibody grafting on the Love wave device using GPTS as coupling agent between SiO2 sensor surface and the sensitive biomembrane layer composed of antibodies. Goat anti-mouse (GAM) antibodies at concentrations from 2μg/ml to 50 μg/ml were introduced on the sensor brass cell. The saturation of the sensor surface by antibodies appears at a concentration of 20 μg/ml. The detection threshold of this primary antibody is around 2 μg/ml, and the lowest concentration allowing antigen detection is around 5 μg/ml.

Keywords

sensoracousticbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 915: Symposium R – Nanostructured Materials and Hybrid Composites for Gas Sensors and Biomedical Applications , 2006 , 0915-R01-08
Copyright
Copyright © Materials Research Society 2006

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