Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-28T17:56:59.125Z Has data issue: false hasContentIssue false
  • English
  • Français

Encapsulation of Urease in Molybdenum Trioxide Sol-Gel

Published online by Cambridge University Press:  01 February 2011

Prashant K. Jha  and
P. I. Gouma
Prashant K. Jha
Affiliation:
Department of Material Science, State University of New York Stony Brook, NY 11794
P. I. Gouma
Affiliation:
Department of Material Science, State University of New York Stony Brook, NY 11794
Get access

Abstract

Molybdenum trioxide sol-gel matrices were tested for their ability to host biomaterials in their structures without adversely affecting the activity of the encapsulated biomolecules. The sol-gel composites were prepared at room temperature; the urease was added during the hydrolysis step to ensure proper encapsulation in the pore structure of the sol. Phosphate buffer solution was added during this stage to maintain the pH value of the sol in the bio-compatible range. The activity of incorporated urease was tested by exposing composite sol-gel material to standard urea test solutions and measuring the amount of gaseous ammonia (released by the reaction of urea with water in the presence of urease) using an ammonia electrode. The concentration of urea solutions ranged from 1mM to 10mM. An electronic olfactory system (EOS 835) was also employed to detect the presence of gaseous ammonia. Gas sensing tests were done to verify the sensitivity of the matrix to ammonia. Microstructural analysis was carried out by means of scanning and transmission electron microscopy. It was found that urease retained its activity inside molybdenum trioxide sol-gels that are sensitive to ammonia. These hybrid nanoporous composites are useful in the field of biosensors and fuel cells.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 828: Symposium A – Semiconductor Materials for Sensing , 2004 , A6.2
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Sampath, S. and Lev, O, Anal. Chem, vol 68, pp. 2015–2021, 1996 Google Scholar
[2] Sheppard, N.F. Jr, Mears, D.J. and Guiseppi-Elie, A, Biosensors and Bioelectronics, vol 11, Iss 10, pp. 967–979, 1996 Google Scholar
[3] Steinschaden, A., Adamovic, D., Jobst, G., Glatz, R., Urban, G., Sensors and Actuators B vol 44, pp. 365–369, 1997 Google Scholar
[4] Soldatkin, A.P., Montoriol, J., Sant, W., Martelet, C., Jaffrezic-renault, N., Biosensors and Bioelectronics, vol 19, Iss 2, pp. 131–135, November 2003 Google Scholar
[5] Watson, L.D., Maynard, P., Cullen, D.C., Sethi, R.S., Brettle, J. and Lowe, C.R., Biosensors, vol 3, pp. 101–115, 1987/1988 Google Scholar
[6] Magalhães, J.M.C.S., Machado, A.A.S.C., Talanta, vol 47, pp. 183–191, 1998 Google Scholar
[7] Lakard, B., Herlem, G., Lakard, S., Antoniou, A., Fahys, B., Biosensors and Bioelectronics, vol 19, pp. 1641–1647, 2004 Google Scholar
[8] Guilbault, G G., Seo, M.L., Talanta, vol 41, Iss 6, pp. 1029–1033, 1994 Google Scholar
[9] Dave, B.C., Dunn, B., Valentine, J. S., Zink, J.I., Anal. Chem, vol 66, pp. 1120–1126, 1994 Google Scholar
[10] Wang, R., Narang, U., Prasad, P.N., Bright, F.V. Anal Chem vol 65, pp 2671–2675, 1993 Google Scholar
[11] Tsai, H., Doong, R., Anal biochem, vol 334, pp 183–192, 2004 Google Scholar
[12] Prasad, A. K., Gouma, P.I., Journal of mat. Sci. vol 38, pp. 4347–4352, 2003 Google Scholar
[13] Dorey, R.C., Draves, J.A., Stanitski., C.L, journal of chemical education vol 76, Iss 6, pp. 752–752, Jun 1999 Google Scholar
[14] Johnson, W.J., Hagge, W.W., Wagoner, R.D., Dinapoli, R.P., Rosevear, J.W. Mayo Clin Proc, vol 47 Iss 1, pp. 21–29, January 1972 Google Scholar