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Electro-kinetically assisted liposomal drug delivery system for characterization of ex-vivo cell-drug interactions

Published online by Cambridge University Press:  06 June 2014

Rajeshwari Taruvai Kalyana Kumar ,
Andi Wangzhou ,
David Kinnamon  and
Shalini Prasad
Rajeshwari Taruvai Kalyana Kumar*
Affiliation:
Department of Bioengineering, University of Texas at Dallas, Richardson, Texas 75080
Andi Wangzhou
Affiliation:
Department of Bioengineering, University of Texas at Dallas, Richardson, Texas 75080
David Kinnamon
Affiliation:
Department of Bioengineering, University of Texas at Dallas, Richardson, Texas 75080
Shalini Prasad
Affiliation:
Department of Bioengineering, University of Texas at Dallas, Richardson, Texas 75080
*
*Email: [email protected]
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Abstract

This work presents a strategy to perform ex-vivo cell-drug interaction studies through electro-kinetically assisted drug delivery system. Here, we present a novel technique to electro-kinetically control the vesicles carrying drug to deliver to pre-determined locations. In order to achieve efficient targeted drug delivery, effect of electrokinetic attractive and repulsive forces on liposomes and target cells were studied and presented. The device consists of a simple bifurcated microfluidic chamber and microelectrodes that assist in carrying the liposomes to the target location. To test the prototype, fully grown human embryonic kidney cell lines (HEK 293) and trypsin as test drug was used. External electrical signal with voltages less than of 5 V peak-to-peak (Vpp) for cells and 10 Vpp for liposomes were applied over a spectrum of frequencies to study the effect of electrokinetic forces. Through this label-free method, we were able to study loading and unloading efficiency of the drug without altering the natural properties of the liposomes and target cells. In this study, characterization and performance comparison studies for two different types of materials (HEK cells and liposomes) were performed. We were able to achieve an overall efficiency of approximately 85%. Various electrical parameters such as applied voltage, frequency and conductivity were manipulated to study the drug-cell interaction. This electrokinetic based method will be highly applicable in understanding the effect on drugs on cell populations ex vivo.

Keywords

cellular (material form)dielectric propertiesfluidics
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1688: Symposium Y – Biomaterials for Biomolecule Delivery and Understanding Cell-Niche Interactions , 2014 , mrss14-1688-y11-04
Copyright
Copyright © Materials Research Society 2014 

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Footnotes

†

Equal contribution

References

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