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Effect of Substrate Surface Modification on Biomineralization of Osteoblasts

Published online by Cambridge University Press:  01 February 2011

Yizhi Meng ,
Xiaolan Ba ,
Seo-Young Kwak ,
Elaine DiMasi ,
Meghan Ruppel ,
Lisa Miller ,
Shouren Ge ,
Nadine Pernodet ,
Miriam Rafailovich  and
Yi-Xian Qin
Yizhi Meng
Affiliation:
[email protected], Stony Brook University, Biomedical Engineering, Psychology A, 3rd Floor, Stony Brook, NY, 11784-2580, United States, 631-632-8607, 631-632-8577
Xiaolan Ba
Affiliation:
[email protected], Stony Brook University, Materials Science & Engineering, Stony Brook, NY, 11794, United States
Seo-Young Kwak
Affiliation:
[email protected], Brookhaven National Laboratory, National Synchrotron Light Source, Upton, NY, 11973, United States
Elaine DiMasi
Affiliation:
[email protected], Brookhaven National Laboratory, National Synchrotron Light Source, Upton, NY, 11973, United States
Meghan Ruppel
Affiliation:
[email protected], Brookhaven National Laboratory, National Synchrotron Light Source, Upton, NY, 11973, United States
Lisa Miller
Affiliation:
[email protected], Brookhaven National Laboratory, National Synchrotron Light Source, Upton, NY, 11973, United States
Shouren Ge
Affiliation:
[email protected], Stony Brook University, Materials Science & Engineering, Stony Brook, NY, 11794, United States
Nadine Pernodet
Affiliation:
[email protected], Stony Brook University, Materials Science & Engineering, Stony Brook, NY, 11794, United States
Miriam Rafailovich
Affiliation:
[email protected], Stony Brook University, Materials Science & Engineering, Stony Brook, NY, 11794, United States
Yi-Xian Qin
Affiliation:
[email protected], Stony Brook University, Biomedical Engineering, Psychology A, 3rd Floor, Stony Brook, NY, 11794-2580, United States
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Abstract

Understanding how biomineralization occurs in the extracellular matrix (ECM) of bone cells is crucial to the development of a successfully engineered bone tissue scaffold, and to date there has not been a well-established method for the quantitative examination of bone mineralization in situ. We investigated the mechanical properties of MC3T3-E1 osteoblast-like cells and the crystalline properties of their biomineralized ECM in vitro using shear modulation force microscopy (SMFM), confocal laser scanning microscopy (CLSM), synchrotron X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The elastic modulus of the mineralizing cells increased at time points corresponding to mineral production, whereas that of the non-mineralizing cells did not vary significantly over time. CLSM showed a restructuring of the F-actin fiber network of mineralizing cells with time, which indicates remodeling activities in the cytoskeleton and was not seen in the non-mineralizing cells. Both XRD and FTIR showed that the mineralizing subclone produced hydroxyapatite in situ and that the non-mineralizing subclone was in fact weakly biomineralizing.

Keywords

bonex-ray diffraction (XRD)infrared (IR) spectroscopy
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D10-09
Copyright
Copyright © Materials Research Society 2007

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References

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