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Effect of Substrate Elasticity on In Vitro Aging of Human Mesenchymal Stem Cells

Published online by Cambridge University Press:  09 January 2013

Courtney E. LeBlon ,
Caitlin R. Fodor ,
Tony Zhang ,
Xiaohui Zhang  and
Sabrina S. Jedlicka
Courtney E. LeBlon
Affiliation:
Mechanical Engineering & Mechanics, Center for Advanced Materials & Nanotechnology Lehigh University, Bethlehem, PA
Caitlin R. Fodor
Affiliation:
Bioengineering Program, Center for Advanced Materials & Nanotechnology Lehigh University, Bethlehem, PA
Tony Zhang
Affiliation:
Bioengineering Program, Center for Advanced Materials & Nanotechnology Lehigh University, Bethlehem, PA
Xiaohui Zhang
Affiliation:
Mechanical Engineering & Mechanics, Center for Advanced Materials & Nanotechnology Lehigh University, Bethlehem, PA
Sabrina S. Jedlicka
Affiliation:
Bioengineering Program, Center for Advanced Materials & Nanotechnology Lehigh University, Bethlehem, PA Materials Science & Engineering, Center for Advanced Materials & Nanotechnology Lehigh University, Bethlehem, PA Center for Advanced Materials & Nanotechnology Lehigh University, Bethlehem, PA
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Abstract

Human mesenchymal stem cells (hMSCs) were routinely cultured on tissue-culture polystyrene (TCPS) to investigate the in vitro aging and cell stiffening. hMSCs were also cultured on thermoplastic polyurethane (TPU), which is a biocompatible polymer with an elastic modulus of approximately 12.9MPa, to investigate the impact of substrate elastic modulus on cell stiffening and differentiation potential. Cells were passaged over several generations on each material. At each passage, cells were subjected to osteogenic and myogenic differentiation. Local cell elastic modulus was measured at every passage using atomic force microscopy (AFM) indentation. Gene and protein expression was examined using qRT-PCR and immunofluorescent staining, respectively, for osteogenic and myogenic markers. Results show that the success of myogenic differentiation is highly reliant on the elastic modulus of the undifferentiated cells. The success of osteogenic differentiations is most likely somewhat dependent on the cell elastic modulus, as differentiations were more successful in earlier passages, when cells were softer.

Keywords

biologicalbiomaterialpolymer
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 39 - 45
Copyright
Copyright © Materials Research Society 2012 

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