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Dielectrophoretical fabrication of hybrid carbon nanotubes-hydrogel biomaterial for muscle tissue engineering applications

Published online by Cambridge University Press:  30 January 2014

Javier Ramón-Azcón ,
Samad Ahadian ,
Raquel Obregon ,
Hitoshi Shiku ,
Ali Khademhosseini  and
Tomokazu Matsue
Javier Ramón-Azcón
Affiliation:
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan
Samad Ahadian
Affiliation:
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan
Raquel Obregon
Affiliation:
Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
Hitoshi Shiku
Affiliation:
Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
Ali Khademhosseini
Affiliation:
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan Department of Medicine, Center for Biomedical Engineering, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA; Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA; Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
Tomokazu Matsue
Affiliation:
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
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Abstract

Dielectrophoresis (DEP) approach was employed to achieve highly aligned multi-walled carbon nanotubes (MWCNTs) within the gelatin methacrylate (GelMA) hydrogels in a facile, rapid, inexpensive, and reproducible manner. This approach enabled us to make different CNTs alignments (e.g., vertical or horizontal alignments) within the GelMA hydrogel using different electrode designs or configurations. Anisotropically aligned GelMA-CNTs hydrogels showed considerably higher conductivity compared to randomly distributed CNTs dispersed in the GelMA hydrogel and the pristine and non-conductive GelMA hydrogel. Adding 0.3 mg/mL CNTs to the GelMA hydrogel led to a slight increase in the mechanical properties of the GelMA and made it to behave as a viscoelastic material. Therefore, it can be used as a suitable scaffold for soft tissues, such as skeletal muscle tissue. 3D microarrays of skeletal muscle myofibers were then fabricated based on the GelMA and GelMA-CNTs hydrogels and they were characterized in terms of gene expressions related to the muscle cell differentiation and contraction. Owing to high electrical conductivity of aligned GelMA-CNTs hydrogels, the engineered muscle tissues cultivated on these materials demonstrated superior maturation and functionality particularly after applying the electrical stimulation (voltage 8 V, frequency 1 Hz, and duration 10 ms for 2 days) compared to the corresponding tissues obtained on the pristine GelMA and randomly distributed CNTs within the GelMA hydrogel.

Keywords

biomaterialelectrical propertiesnanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 81 - 86
Copyright
Copyright © Materials Research Society 2014 

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References

REFERENCES

Slaughter, B V, Khurshid, SS, Fisher, OZ, Khademhosseini, A, Peppas, NA., Adv. Mater. 21, 3307 (2009).CrossRefGoogle Scholar
Peppas, NA, Hilt, JZ, Khademhosseini, A, Langer, R., Adv. Mater. 18, 1345 (2006).CrossRefGoogle Scholar
Dvir, T, Timko, BP, Kohane, DS, Langer, R. Nat., Nanotechnol. 6, 13 (2011).CrossRefGoogle Scholar
Dvir, T, Timko, BP, Brigham, MD, Naik, SR, Karajanagi, SS, Levy, O, et al. ., Nat. Nanotechnol. 6, 720 (2011).CrossRefGoogle Scholar
You, J-O, Rafat, M, Ye, GJC, Auguste, DT., Nano Lett. 11, 3643 (2011).CrossRefGoogle Scholar
Stout, DA, Basu, B, Webster, TJ., Acta Biomater. 7, 3101 (2011).CrossRefGoogle Scholar
Nichol, JW, Koshy, ST, Bae, H, Hwang, CM, Yamanlar, S, Khademhosseini, A, Biomaterials 31, 5536 (2010).CrossRefGoogle Scholar
Aubin, H, Nichol, JW, Hutson, CB, Bae, H, Sieminski, AL, Cropek, DM, et al. ., Biomaterials 31, 6941 (2010).CrossRefGoogle Scholar
MacDonald, RA, Voge, CM, Kariolis, M, Stegemann, JP, Acta Biomater. 4, 1583 (2008).CrossRefGoogle Scholar
Bal, S., Materials and Design 31, 2406 (2010).CrossRefGoogle Scholar
Wang, B, Ma, Y, Li, N, Wu, Y, Li, F, Chen, Y, Adv. Mater. 22, 3067 (2010).CrossRefGoogle Scholar
Ramón-Azcón, J, Yasukawa, T, Lee, HJ, Matsue, T, Sánchez-Baeza, F, Marco, M-P, et al. ., Biosens. Bioelectron. 25, 1928 (2010).CrossRefGoogle Scholar
Ramón-Azcón, J, Kunikata, R, Sanchez, F-J, Marco, M-P, Shiku, H, Yasukawa, T, et al. ., Biosens. Bioelectron. 24, 1592 (2009).CrossRefGoogle Scholar
Voge, CM, Johns, J, Raghavan, M, Morris, MD, Stegemann, JP, J. Biomed. Mater. Res. A 101, 231 (2013).CrossRefGoogle Scholar
Melechko, Anatoli V, Desikan, Ramya, McKnight, Timothy E, Klein, Kate L, Rack, Philip D., J. Phys. D App. Phys. 42, 193001 (2009).CrossRefGoogle Scholar
Nguyen-Vu, TDB, Chen, H, Cassell, AM, Andrews, R, Meyyappan, M, Li, J., Small. 2, 89 (2006).CrossRefGoogle Scholar