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Develoapmet of a Tissue Analog for Cartilage Repair

Published online by Cambridge University Press:  15 February 2011

J. M. Pachence ,
S. R. Frenkel  and
H. Lin
J. M. Pachence
Affiliation:
(ABS LifeSciences, Inc., West Chester, PA)
S. R. Frenkel
Affiliation:
(Hospital for Joint Diseases/orthoaedic Institute, New York, NY).
H. Lin
Affiliation:
(Hospital for Joint Diseases/orthoaedic Institute, New York, NY).
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Abstract

Purified type I collagen was formed into matrices whose pore sizes were defined on the basis of previous results. The first series of in vitro studies measured the metabolism of chondrocytes grown in matrices with various pore sizes; results revealed that the growth rate was independent of the average matrix pore size, but that ckmdrocyte infiltration throughout the matrix was optimal for pore sizes of 100 to 150 un. In a second series of studies, type I collagen was combined with hyaluranic acid; the HyA/collagen matrices had little effect on chcrdrocyte cell growth versus the collagen matrices. A third set of in vitro studies used collagen matrices incorporating varying cornentrations of insulin-like growth factor. It was found that the IGF-1/collagen matrices can significantly effect the growth and metabolism of the clxrihrocytes. These experiments were vital in establishing the collagen matrix parameters which will be used in subsequent in vivo studies.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 125
Copyright
Copyright © Materials Research Society 1992

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References

REFERENCES

1. Campbell, C.J., Clin. Ortlq. Rel. Res. 64, 45–63 (1969).Google Scholar
2. Manning, W., and Bcnner, W., Arthr. Rheulm. 10, 235–239 (1967).Google Scholar
3. Sokoloff, L., In: The Joints and Synoviol Fluid (Vol. II), edited by Sokoloff, L. (Academic Press, New York, 1978), pp. 1–27 Google Scholar
4. DePalma, A., Tsa-Hos, T., and Maaler, G., J. Bone Joint Surg. 45A, 1565–1578 (1963).Google Scholar
5. Selignan, G., Clin. Orthop. Rel. Res. 87, 332–348 (1972).Google Scholar
6. Green, W.T., Clin. Orthop. Rel. Res. 124, 237–250 (1977).Google Scholar
7. Bentley, G., Smith, A.V., and Mukerjhee, R., Ann. Rheum. Dis. 37, 449–458 (1978).Google Scholar
8. Bloom, W., and Fawett, D., In: A Textbook of Histology, 10th Edition(W.B. Saunders Co., Philadelphia, PA, 1975).Google Scholar
9. Grande, D., Singh, I., and Pugh, J., Anat. Rec. 218, 142–148 (1987).Google Scholar
10. Grande, D., Pitman, M.I., Peterson, L., Menzhe, D., and Klein, M., J. Ortho. Res. 7, 208–218 (1989).Google Scholar
11. Freshney, R.I., In: Culture of Animal Cells: A Manual of Basic Techniques (Alan R. Liss, Inc., New York, 1983), p. 208.Google Scholar
12. Kim, Y.J., Sah, R., Doong, J.Y., and Grodzinsky, A.J., Analyt. Biochem. (1986)Google Scholar
13. Tcama, E.A. and Pavelec, M., J. Gercntology 26, 310–315 (1971).Google Scholar
14. Itay, S., Abramovici, A., and Nevo, Z., Clin. Ortho. Rel. Res., 220, 284–303 (1987).Google Scholar
15. Farndale, R., Buttle, D., and Barrett, A., Biochim. Bicphys. Acta 883, 173–177 (1986).Google Scholar
16. Doillon, C.J., VhrIne, C.F., Brandin, S., and Silver, F.H., J. Biamed. Mater. Res. 20, 1219–1228 (1986).CrossRefGoogle Scholar
17. Li, S.T., Soc. Neurosci. Absts. 13, 291.8 (1987)Google Scholar