Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-24T16:41:26.088Z Has data issue: false hasContentIssue false
  • English
  • Français

Demonstration of Molecular Sensing Using QCM Device Coated with Stimuli-sensitive Hydrogel

Published online by Cambridge University Press:  29 November 2012

Yoshimi. Seida ,
Yuri Nakano  and
Yoshio Nakano
Yoshimi. Seida
Affiliation:
Natural Science Laboratory, Toyo University, Tokyo, 112-8606 Japan Interdisciplinary Graduate School of Science and Technology, Tokyo Institute of Technology, Yokohama, 226-8504 Japan
Yuri Nakano
Affiliation:
Interdisciplinary Graduate School of Science and Technology, Tokyo Institute of Technology, Yokohama, 226-8504 Japan
Yoshio Nakano
Affiliation:
Interdisciplinary Graduate School of Science and Technology, Tokyo Institute of Technology, Yokohama, 226-8504 Japan
Get access

Abstract

Molecular sensing using stimuli-responsive viscoelastic property of amphiphilic polymer hydrogel was demonstrated. Thermo-responsive poly(N-isopropylacrylamide); NIPA, hydrogel immobilizing bovine serum albumin (BSA) in its polymer chain was synthesized on the AT-cut QCM (Quartz crystal microbalance). The device was provided to the sensing of anti-BSA antibody. The resonance behavior of QCM in response to the adsorption of anti-BSA antibody on the hydrogel was observed based on both the resonance frequency and resistance of QCM with impedance analysis. The QCM device coated with the LCST polymer hydrogel revealed a characteristic resonance behavior in response to the adsorption of anti-BSA antibody in the collapse phase of gel at which the gel lost most of its hydrated water. The resonance frequency and resistance in the collapsed gel were highly sensitive and their changes in response to the target adsorption were much larger than in the swelling state of gel. The use of the device at the LCST of NIPA gel is ideal because the largest amplification of the adsorption signal is available along with the largest phase change of NIPA gel.

Keywords

adsorptionsensormicrostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 151 - 156
Copyright
Copyright © Materials Research Society 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Seida, Y., Sato, I., Taki, K. and Nakano, Y., Trans. Mat. Res. Soc., 32, 783 (2007)Google Scholar
Paul, S., paul, D., Basova, T., and Ray, A. K., J. Phys. Chem. C, 112, 11822(2008)CrossRefGoogle Scholar
Shoemaker, S. G., Hoffman, A. S., and Priest, J. H., Applied Biochemistry and Biotechnology, 15, 11(1987)CrossRefGoogle Scholar
Miyata, T., Asami, N., and Uragami, T., Nature, 399, 766 (1999 a)CrossRefGoogle Scholar
Lu, Z. R., Kopeckova, P., and Kopecek, J., Macromol. Biosci., 3, 296(2003)CrossRefGoogle Scholar
Aitkan, A. and Learmonth, M. P.; “Protein Determination by UV Absorption,” The Protein Protocols Hand Book, 2 nd Edition, Humana Press Inc., Totowa, NJ, 3–5 (2002)CrossRefGoogle Scholar
Seida, Y. and Nakano, Y., J. Chem. Eng. Japan, 29, 767 (1996)CrossRefGoogle Scholar
Kanazawa, K. and Gordon, J. G., Anal. Chim. Acta, 175, 99 (1985)CrossRefGoogle Scholar
Seida, Y., Proc. 10th Japan-Korea Symposium on Materials & Interfaces, Proc. Int. Symposium on Frontiers in Chemical Engineering, Kyoto, 16(2012)Google Scholar
Sauerbrey, G., Z. Für. Phys., 155, 206 (1959)CrossRefGoogle Scholar
Nakano, Y., Seida, Y. and Nakano, Y., J. Chem. Eng. Japan, 42(7), 531(2009)CrossRefGoogle Scholar