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Composites and Structures for Regenerative Engineering

Published online by Cambridge University Press:  07 January 2014

Cato T. Laurencin  and
Roshan James
Cato T. Laurencin*
Affiliation:
Institute for Regenerative Engineering, The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030, U.S.A The Raymond and Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030, U.S.A Connecticut Institute for Clinical and Translational Science, The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030, U.S.A Department of Orthopaedic Surgery, The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030, U.S.A
Roshan James
Affiliation:
Institute for Regenerative Engineering, The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030, U.S.A The Raymond and Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030, U.S.A Department of Orthopaedic Surgery, The University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030, U.S.A
*
*Corresponding author: Cato T. Laurencin, M.D.,Ph.D. University Professor University of Connecticut Health Center 263 Farmington Avenue, Farmington, CT, USA 06030 Phone: +1-860-679-6544 Fax: +1-860-679-1553 Email: [email protected]
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Abstract

Regenerative engineering was conceptualized by bridging the lessons learned in developmental biology and stem cell science with biomaterial constructs and engineering principles to ultimately generate de novo tissue. We seek to incorporate our understanding of natural tissue development to design tissue-inducing biomaterials, structures and composites than can stimulate the regeneration of complex tissues, organs, and organ systems through location-specific topographies and physico-chemical cues incorporated into a continuous phase. This combination of classical top-down tissue engineering approach with bottom-up strategies used in regenerative biology represents a new multidisciplinary paradigm. Advanced surface topographies and material scales are used to control cell fate and the consequent regenerative capacity.

Musculoskeletal tissues are critical to the normal functioning of an individual and following damage or degeneration they show extremely limited endogenous regenerative capacity. The increasing demand for biologically compatible donor tissue and organ transplants far outstrips the availability leading to an acute shortage. We have developed several biomimetic structures using various biomaterial platforms to combine optimal mechanical properties, porosity, bioactivity, and functionality to effect repair and regeneration of hard tissues such as bone, and soft tissues such as ligament and tendon. Starting with simple structures, we have developed composite and multi-scale systems that very closely mimic the native tissue architecture and material composition. Ultimately, we aim to modulate the regenerative potential, including proliferation, phenotype maturation, matrix production, and apoptosis through cell-scaffold and host –scaffold interactions developing complex tissues and organ systems.

Keywords

biomaterialcompositenanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 3 - 15
Copyright
Copyright © Materials Research Society 2014 

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References

REFERENCES

Vacanti, J. P., “Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation,” Lancet, vol. 354, p. S32, 1999.CrossRefGoogle ScholarPubMed
Fung, Y., “A proposal to the National science Foundation for An Engineering Research Centre at USCD,” Center for the Engineering of Living Tissues. UCSD, vol. 865023, 2001.Google Scholar
Bauer, T. W. and Muschler, G. F., “Bone graft materials: an overview of the basic science,” Clinical orthopaedics and related research, vol. 371, p. 10, 2000.CrossRefGoogle Scholar
Langer, R. and Vacanti, J., “Tissue engineering,” Science, vol. 260, pp. 920-926, May 14, 1993 1993.CrossRefGoogle ScholarPubMed
Laurencin, C. T. and Khan, Y., “Regenerative Engineering,” Science Translational Medicine, vol. 4, p. 160ed9, November 14, 2012 2012.CrossRefGoogle ScholarPubMed
James, R., Daley, G. Q., and Laurencin, C. T., “Regenerative Engineering: Materials, Mimicry, and Manipulations to Promote Cell and Tissue Growth,” National Academy of Engineering - The Bridge: The Convergence of Engineering and the Life Sciences; Editors: Philip A. Sharp and Robert Langer, vol. 43, p. 8, 2013.Google Scholar
Reichert, W., Ratner, B. D., Anderson, J., Coury, A., Hoffman, A. S., Laurencin, C. T., et al. ., “2010 Panel on the biomaterials grand challenges,” Journal of Biomedical Materials Research Part A, vol. 96, pp. 275–287, 2011.CrossRefGoogle ScholarPubMed
Sharp, P. A. and Langer, R., “Promoting Convergence in Biomedical Science,” Science, vol. 333, p. 527, July 29, 2011 2011.CrossRefGoogle ScholarPubMed
Peerani, R., Rao, B. M., Bauwens, C., Yin, T., Wood, G. A., Nagy, A., et al. ., “Niche-mediated control of human embryonic stem cell self-renewal and differentiation,” EMBO J, vol. 26, pp. 4744–55, Nov 14 2007.CrossRefGoogle ScholarPubMed
Petersen, T. H., Calle, E. A., Zhao, L., Lee, E. J., Gui, L., Raredon, M. B., et al. ., “Tissue-Engineered Lungs for in Vivo Implantation,” Science, vol. 329, pp. 538-541, July 30, 2010 2010.CrossRefGoogle ScholarPubMed
Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., et al. ., “Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart,” Nature medicine, vol. 14, pp. 213–221, 2008.CrossRefGoogle Scholar
Badylak, S. F., Taylor, D., and Uygun, K., “Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds,” Annu Rev Biomed Eng, vol. 13, pp. 27–53, Aug 15 2011.CrossRefGoogle ScholarPubMed
Dvir, T., Timko, B. P., Kohane, D. S., and Langer, R., “Nanotechnological strategies for engineering complex tissues,” Nat Nanotechnol, vol. 6, pp. 13–22, Jan 2011.CrossRefGoogle ScholarPubMed
Kelleher, C. M. and Vacanti, J. P., “Engineering extracellular matrix through nanotechnology,” J R Soc Interface, vol. 7 Suppl 6, pp. S717–29, Dec 6 2010.CrossRefGoogle ScholarPubMed
James, R., Kesturu, G., Balian, G., and Chhabra, A. B., “Tendon: Biology, biomechanics, repair, growth factors, and evolving treatment options,” Journal of Hand Surgery-American Volume, vol. 33A, pp. 102–112, Jan 2008.CrossRefGoogle Scholar
James, R., Deng, M., Laurencin, C., and Kumbar, S., “Nanocomposites and bone regeneration,” Frontiers of Materials Science, vol. 5, pp. 342–357, 2011.CrossRefGoogle Scholar
Hogan, M. V., Bagayoko, N., James, R., Starnes, T., Katz, A., and Chhabra, A. B., “Tissue engineering solutions for tendon repair,” J Am Acad Orthop Surg, vol. 19, pp. 134–42, Mar 2011.CrossRefGoogle ScholarPubMed
Meng, D., James, R., Laurencin, C. T., and Kumbar, S. G., “Nanostructured Polymeric Scaffolds for Orthopaedic Regenerative Engineering,” NanoBioscience, IEEE Transactions on, vol. 11, pp. 3–14, 2012.Google Scholar
Borden, M., Attawia, M., Khan, Y., and Laurencin, C. T., “Tissue engineered microsphere-based matrices for bone repair::: design and evaluation,” Biomaterials, vol. 23, pp. 551–559, 2002.CrossRefGoogle ScholarPubMed
Borden, M., El-Amin, S., Attawia, M., and Laurencin, C., “Structural and human cellular assessment of a novel microsphere-based tissue engineered scaffold for bone repair,” Biomaterials, vol. 24, pp. 597–609, 2003.CrossRefGoogle ScholarPubMed
Laurencin, C., Attawia, M., Lu, L., Borden, M., Lu, H., Gorum, W., et al. ., “Poly (lactide-co-glycolide)/hydroxyapatite delivery of BMP-2-producing cells: a regional gene therapy approach to bone regeneration,” Biomaterials, vol. 22, pp. 1271–1277, 2001.CrossRefGoogle ScholarPubMed
Roveri, N., Falini, G., Sidoti, M., Tampieri, A., Landi, E., Sandri, M., et al. ., “Biologically inspired growth of hydroxyapatite nanocrystals inside self-assembled collagen fibers,” Materials Science and Engineering: C, vol. 23, pp. 441–446, 2003.CrossRefGoogle Scholar
Webster, T. J., Ergun, C., Doremus, R. H., Siegel, R. W., and Bizios, R., “Enhanced functions of osteoblasts on nanophase ceramics,” Biomaterials, vol. 21, pp. 1803–1810, 2000.CrossRefGoogle ScholarPubMed
Webster, T. J., Ergun, C., Doremus, R. H., Siegel, R. W., and Bizios, R., “Enhanced osteoclast-like cell functions on nanophase ceramics,” Biomaterials, vol. 22, pp. 1327–1333, 2001.CrossRefGoogle ScholarPubMed
Webster, T. J., Siegel, R. W., and Bizios, R., “Osteoblast adhesion on nanophase ceramics,” Biomaterials, vol. 20, pp. 1221–1227, 1999.CrossRefGoogle ScholarPubMed
Lv, Q., Nair, L., and Laurencin, C. T., “Fabrication, characterization, and in vitro evaluation of poly(lactic acid glycolic acid)/nano-hydroxyapatite composite microsphere-based scaffolds for bone tissue engineering in rotating bioreactors,” Journal of Biomedical Materials Research Part A, vol. 91A, pp. 679–691, 2009.CrossRefGoogle Scholar
Kumbar, S. G., James, R., Nukavarapu, S. P., and Laurencin, C. T., “Electrospun nanofiber scaffolds: engineering soft tissues,” Biomedical materials, vol. 3, p. 034002, Sep 2008.CrossRefGoogle ScholarPubMed
Kumbar, S. G., Kofron, M. D., Nair, L. S., and Laurencin, C. T., “Cell Behavior Toward Nanostructured Surfaces,” in Biomedical Nanostructures, Gonsalves KE, H. C., Laurencin, CT, Nair LS, Ed., ed Hoboken, NJ, USA: John Wiley & Sons, 2007, pp. 261–295.CrossRefGoogle Scholar
Li, W. J., Laurencin, C. T., Caterson, E. J., Tuan, R. S., and Ko, F. K., “Electrospun nanofibrous structure: a novel scaffold for tissue engineering,” J Biomed Mater Res, vol. 60, pp. 613–21, Jun 15 2002.CrossRefGoogle ScholarPubMed
Nair, L. S., Bhattacharyya, S., Bender, J. D., Greish, Y. E., Brown, P. W., Allcock, H. R., et al. ., “Fabrication and optimization of methylphenoxy substituted polyphosphazene nanofibers for biomedical applications,” Biomacromolecules, vol. 5, pp. 2212–20, 2004.CrossRefGoogle ScholarPubMed
Bhattacharyya, S., Nair, L. S., Singh, A., Krogman, N. R., Greish, Y. E., Brown, P. W., et al. ., “Electrospinning of poly[bis(ethyl alanato) phosphazene] nanofibers “ J Biomed Nanotechnol, vol. 2, pp. 36–45, 2006.CrossRefGoogle Scholar
Bhattacharyya, S., Kumbar, S. G., Khan, Y. M., Nair, L. S., Singh, A., Krogman, N. R., et al. ., “Biodegradable polyphosphazene-nanohydroxyapatite composite nanofibers: scaffolds for bone tissue engineering,” J Biomed Nanotechnol, vol. 5, pp. 69–75, 2009.CrossRefGoogle ScholarPubMed
Li, W. J., Laurencin, C. T., Caterson, E. J., Tuan, R. S., and Ko, F. K., “Electrospun nanofibrous structure: A novel scaffold for tissue engineering,” Journal of Biomedical Materials Research, vol. 60, pp. 613–621, Jun 2002.CrossRefGoogle ScholarPubMed
Christenson, E. M., Anseth, K. S., van den Beucken, J. J., Chan, C. K., Ercan, B., Jansen, J. A., et al. ., “Nanobiomaterial applications in orthopedics,” J Orthop Res, vol. 25, pp. 11–22, 2007.CrossRefGoogle ScholarPubMed
Nair, L. S. and Laurencin, C. T., “Nanofibers and nanoparticles for orthopaedic surgery applications,” J Bone Joint Surg Am, vol. 90, pp. 128–131, 2008.CrossRefGoogle ScholarPubMed
Nair, L. S., Bhattacharyya, S., and Laurencin, C. T., “Development of novel tissue engineering scaffolds via electrospinning,” Expert Opin Biol Ther, vol. 4, pp. 659–668, 2004.CrossRefGoogle ScholarPubMed
Woo, K. M., Chen, V. J., and Ma, P. X., “Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment,” J Biomed Mater Res A, vol. 67A, pp. 531–537, 2003.CrossRefGoogle Scholar
Pelled, G., Tai, K., Sheyn, D., Zilberman, Y., Kumbar, S., Nair, L. S., et al. ., “Structural and nanoindentation studies of stem cell-based tissue-engineered bone,” J Biomech, vol. 40, pp. 399–411, 2007.CrossRefGoogle ScholarPubMed
Conconi, M. T., Lora, S., Menti, A. M., Carampin, P., and Parnigotto, P. P., “In vitro evaluation of poly[bis(ethyl alanato)phosphazene] as a scaffold for bone tissue engineering,” Tissue Eng, vol. 12, pp. 811–9, Apr 2006.CrossRefGoogle ScholarPubMed
Deng, M., Kumbar, S. G., Nair, L. S., Weikel, A. L., Allcock, H. R., and Laurencin, C. T., “Biomimetic structures: biological implications of dipeptide-substituted polyphosphazene-polyester blend nanofiber matrices for load-bearing bone regeneration ” Adv Funct Mater, vol. 21, pp. 2641–2651, 2011.CrossRefGoogle Scholar
Cooper, J. A. Jr., Sahota, J. S., Gorum, W. J. II, Carter, J., Doty, S. B., and Laurencin, C. T., “Biomimetic tissue-engineered anterior cruciate ligament replacement,” Proc Natl Acad Sci U S A, vol. 104, pp. 3049–3054, 2007.CrossRefGoogle ScholarPubMed
Peach, M. S., Roshan, J., Udaya, S. T., Meng, D., Nicole, L. M., Harry, R. A., et al. ., “Polyphosphazene functionalized polyester fiber matrices for tendon tissue engineering: in vitro evaluation with human mesenchymal stem cells,” Biomedical materials, vol. 7, p. 045016, 2012.CrossRefGoogle ScholarPubMed
Peach, M. S., Kumbar, S. G., James, R., Toti, U. S., Balasubramaniam, D., Deng, M., et al. ., “Design and optimization of polyphosphazene functionalized fiber matrices for soft tissue regeneration,” J Biomed Nanotechnol, vol. 8, pp. 107–24, 2012.CrossRefGoogle ScholarPubMed
James, R., Toti, U. S., Laurencin, C. T., and Kumbar, S. G., “Electrospun Nanofibrous Scaffolds for Engineering Soft Connective Tissues,” Methods Mol Biol, vol. 726, pp. 243–258, 2011.CrossRefGoogle ScholarPubMed