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Characterization of Crustacyanin-A2 Subunit as a Component of the Organic Matrix of Gastroliths from the Crayfish Cherax quadricarinatus

Published online by Cambridge University Press:  31 January 2011

Gilles Luquet ,
Nathalie Le Roy ,
Isabelle Zanella-Cléon ,
Michel Becchi ,
Sergio Bucarey ,
Maria Soledad Fernandez ,
Jose Luis Arias ,
Nathalie Guichard ,
Benjamin Marie  and
Frédéric Marin
Gilles Luquet
Affiliation:
[email protected], Université de Bourgogne, UMR CNRS 5561 Biogéosciences, Dijon, France
Nathalie Le Roy
Affiliation:
[email protected], Université de Bourgogne, UMR CNRS 5561 Biogéosciences, Dijon, France
Isabelle Zanella-Cléon
Affiliation:
[email protected], Université Lyon 1 - Institut de Biologie et Chimie des Protéines, UMR CNRS 5086 Laboratoire de Spectrométrie de Masse, Lyon, France
Michel Becchi
Affiliation:
[email protected], Université Lyon 1 - Institut de Biologie et Chimie des Protéines, UMR CNRS 5086 Laboratoire de Spectrométrie de Masse, Lyon, France
Sergio Bucarey
Affiliation:
[email protected], University of Chile, Faculty of Veterinary and Animal Sciences and Centre for Advanced Interdisciplinary Research in Materials (CIMAT), Santiago de Chile, Chile
Maria Soledad Fernandez
Affiliation:
[email protected], University of Chile, Faculty of Veterinary and Animal Sciences and Centre for Advanced Interdisciplinary Research in Materials (CIMAT), Santiago de Chile, Chile
Jose Luis Arias
Affiliation:
[email protected], University of Chile, Faculty of Veterinary and Animal Sciences and Centre for Advanced Interdisciplinary Research in Materials (CIMAT), Santiago de Chile, Chile
Nathalie Guichard
Affiliation:
[email protected], Université de Bourgogne, UMR CNRS 5561 Biogéosciences, Dijon, France
Benjamin Marie
Affiliation:
[email protected], Université de Bourgogne, UMR CNRS 5561 Biogéosciences, Dijon, France
Frédéric Marin
Affiliation:
[email protected], United States
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Abstract

Like the lobsters, some terrestrial crabs and other crayfishes, the Australian red claw crayfish, Cherax quadricarinatus, elaborates in its stomach wall calcium storage structures called gastroliths. For understanding the cyclic elaboration and stabilization of these amorphous calcified structures, we studied the organic matrix (OM) of these paired biomineralizations. After decalcification with acetic acid, we analysed the proteinaceous components of an acetic acid-insoluble fraction by two-dimensional electrophoresis. Nine spots were digested by trpsin and the tryptic peptides were sequenced by nanoLC-nanoESI-MS/MS mass spectrometry. About 100 peptidic sequences were compared to sequences previously registered in the databases. Seven of the partially sequenced organic matrix polypeptides are probably new proteins. Another one corresponds to the previously sequenced protein, GAP65, from Cherax quadricarinatus and the last one, which migrates in electrophoresis at around 25 kDa, presents strong homology with the crustacyanin-A2 subunit of Homarus gammarus.

Keywords

mass spectroscopyamorphousstorage
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1187: Symposium KK – Structure-Property Relationships in Biomineralized and Biomimetic Composites , 2009 , 1187-KK05-03
Copyright
Copyright © Materials Research Society 2009

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