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Anisotropic Cellular Alignment on Nano-Wrinkled Polymeric Surface

Published online by Cambridge University Press:  12 January 2012

Toshinori Fujie ,
Francesco Greco ,
Silvia Taccola ,
Leonardo Ricotti ,
Arianna Menciassi  and
Virgilio Mattoli
Toshinori Fujie
Affiliation:
Center for MicroBioRobotics IIT@SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy European Biomedical Science Institute (EBSI), Organization for European Studies, Waseda University, 2-2 Wakamtsu-cho, Shinjuku, Tokyo 162-8480, Japan
Francesco Greco
Affiliation:
Center for MicroBioRobotics IIT@SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy
Silvia Taccola
Affiliation:
Center for MicroBioRobotics IIT@SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy Biorobotics Institute, Scuola Superiore Sant’Anna, Polo Sant’Anna Valdera, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy
Leonardo Ricotti
Affiliation:
Biorobotics Institute, Scuola Superiore Sant’Anna, Polo Sant’Anna Valdera, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy
Arianna Menciassi
Affiliation:
Center for MicroBioRobotics IIT@SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy Biorobotics Institute, Scuola Superiore Sant’Anna, Polo Sant’Anna Valdera, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy
Virgilio Mattoli
Affiliation:
Center for MicroBioRobotics IIT@SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy
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Abstract

Convenient preparation of nano/micro scale topography is crucial for the fabrication of low-cost biodevices, which could be useful tools for understanding cell biology mechanisms and for the development of scaffolds for tissue engineering. Such intelligent surfaces have been conventionally fabricated through photolithography, micro-contact printing, and nano/micro imprinting. However, considering the process integration, these approaches are not always adequate in order to produce large dimensional patterns in a convenient and rapid way. In this study, we focused on the convenient fabrication of nano-wrinkles based on the elastic instability between a shape memory polymer sheet and a conductive polymeric film, on which the behavior of murine skeletal muscle cells (C2C12) was evaluated. A tens-of-nm-thick layer of poly(3,4-ethylenedioxythiophene) with poly(styrenesulfonate) (PEDOT:PSS) was spincoated on a thermo-retractable polymer sheet. Then, thermal treatment produced different periodicity of the unidirectional nano-wrinkles on the polymer sheet covered with different thickness of PEDOT:PSS layer. Finally, adhesion and proliferation of C2C12 were evaluated, comparing different samples. The cells preferentially adhered and anisotropically aligned on low and narrow ridges (1.5 μm height) rather than on high and wide ones (2.5 μm height). Furthermore, we observed that these trends were confirmed in the differentiation stage of C2C12 into myotubes. The combination of living cells and tunable nano-wrinkles made of conductive polymeric materials will represent a unique tool for the development of innovative biomedical devices.

Keywords

biomimetic (assembly)cellular (material form)nanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1415: Symposium HH/II/TT – MEMS, BioMEMS and Bioelectronics–Materials and Devices , 2012 , mrsf11-1415-ii09-02
Copyright
Copyright © Materials Research Society 2012

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References

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