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Aminopropyl Embedded Silica Films as Potent Substrates in DNA Microarray Applications

Published online by Cambridge University Press:  01 February 2011

Kristjan Saal ,
Margo Plaado ,
Ilmar Kink ,
Ants Kurg ,
Valter Kiisk ,
Jevgenia Koževnikova ,
Uno Mäeorg ,
Ago Rinken ,
Ilmo Sildos  and
Tanel Tätte
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Kristjan Saal
Affiliation:
Institute of Physics, University of Tartu, 142 Riia St., 51014 Tartu, Estonia Institute of Organic and Bioorganic Chemistry, University of Tartu, 2 Jakobi St., 51014 Tartu, Estonia
Margo Plaado
Affiliation:
Institute of Physics, University of Tartu, 142 Riia St., 51014 Tartu, Estonia Institute of Organic and Bioorganic Chemistry, University of Tartu, 2 Jakobi St., 51014 Tartu, Estonia
Ilmar Kink
Affiliation:
Institute of Physics, University of Tartu, 142 Riia St., 51014 Tartu, Estonia
Ants Kurg
Affiliation:
Institute of Molecular and Cell Biology, Estonian Biocentre, 23 Riia St., 51010 Tartu, Estonia
Valter Kiisk
Affiliation:
Institute of Physics, University of Tartu, 142 Riia St., 51014 Tartu, Estonia
Jevgenia Koževnikova
Affiliation:
Institute of Molecular and Cell Biology, Estonian Biocentre, 23 Riia St., 51010 Tartu, Estonia
Uno Mäeorg
Affiliation:
Institute of Organic and Bioorganic Chemistry, University of Tartu, 2 Jakobi St., 51014 Tartu, Estonia
Ago Rinken
Affiliation:
Institute of Organic and Bioorganic Chemistry, University of Tartu, 2 Jakobi St., 51014 Tartu, Estonia
Ilmo Sildos
Affiliation:
Institute of Physics, University of Tartu, 142 Riia St., 51014 Tartu, Estonia
Tanel Tätte
Affiliation:
Institute of Physics, University of Tartu, 142 Riia St., 51014 Tartu, Estonia Institute of Organic and Bioorganic Chemistry, University of Tartu, 2 Jakobi St., 51014 Tartu, Estonia
Ants Lõhmus
Affiliation:
Institute of Physics, University of Tartu, 142 Riia St., 51014 Tartu, Estonia
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Abstract

Sol-gel derived silane hybrid films composing of (3-aminopropyl)trimethoxysilane–tetramethoxysilane (APTMS-TMOS) have been shown to have properties that make the films suitable for DNA microarray applications. The ability of the films to bind DNA was studied using aminated 25-mer oligonucleotide DNA and 1, 4-phenylenediisothiocyanate linker. The binding of DNA onto the films was shown to depend on films' composition i.e. the binding rate decreased with the decrease of APTMS content in the film. The arrayed primer extension (APEX) analysis showed reversed tendency – fluorescence signals of bound probes increased with the decrease of APTMS content in the film. The background noise was low and the fluorescent probes were clearly outlined. The density of functional amino groups on the films was estimated by fluorescence spectroscopy.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 873: Symposium K – Biological and Bio-Inspired Materials and Devices , 2005 , K9.3
Copyright
Copyright © Materials Research Society 2005

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