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3-Mercaptopropionic Acid modified Porous Silicon Substrate used in Hyperammonemia

Published online by Cambridge University Press:  01 February 2011

Dong-hwa Yun ,
Jun-Hyoung Chang ,
Woo-Jin Lee  and
Suk-In Hong
Dong-hwa Yun
Affiliation:
[email protected], Korea University, Chemical & Biological Engineering, Anam-dong Seongbuk-Gu, Seoul, 136-701, Korea, Republic of, 82-2-3290-3722
Jun-Hyoung Chang
Affiliation:
[email protected], Korea University, Chemical & Biological Engineering, Anam-dong Seongbuk-Gu, Seoul, 136-701, Korea, Republic of
Woo-Jin Lee
Affiliation:
[email protected], Korea University, Chemical & Biological Engineering, Anam-dong Seongbuk-Gu, Seoul, 136-701, Korea, Republic of
Suk-In Hong
Affiliation:
[email protected], Korea University, Chemical & Biological Engineering, Anam-dong Seongbuk-Gu, Seoul, 136-701, Korea, Republic of
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Abstract

Amperometric urea sensor is more suitable than optical and potentiometric urea sensor to diagnose hyperammonemia. However, because sensitivity in low concentration decreases remarkably, despite amperometric urea sensor has been studied for a long time it has not been applied for clinical diagnosis. In this paper, a new structure for an amperometric urea sensor was fabricated by MEMS, electrochemical etching, and electrostatic covalent binding techniques. Until now most amperometric urea sensors have had a membrane fixed on top of the transducer. That method often leads to malfunction of the sensor, arising from problems such as inadequate membrane adhesion, insufficient mechanical stability, and low sensitivity. To solve these kinds of problems, urease (Urs) was immobilized by electrostatic covalent binding method on the porous silicon (PSi) substrate coated self-assembled monolayer (SAM). Electrostatic covalent binding method was used to keep anisotropic orientation of urease on SAM.

Keywords

self-assemblysensorSi
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1063: Symposium OO – Solids at the Biological Interface , 2007 , 1063-OO06-09
Copyright
Copyright © Materials Research Society 2008

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References

REFERENCES

1. Senillou, A., Jaffrezic-Renault, A., Martelet, C. and Cosnier, S., Talanta 50, 219 (1999).Google Scholar
2. Koncki, R., Radomska, A. and Glab, S., Talanta 52, 13 (2002).Google Scholar
3. Milardovic, S., Kruhak, I. and Grabaric, B. S., Laboratory Robotics and Automation 11, 266 (1999).Google Scholar
4. Lobanov, O. V., Dubrovsky, T. B., Savitsky, A. P., Akindinov, D. E., Alexeev, I. V. and Savransky, V. V., Thin Solid Films 259, 85 (1995).Google Scholar
5. Malitesta, C., F. Palmisano and Torsi, L., Analytical Chemistry 62, 2735 (1990).Google Scholar
6. Campanella, L., Mazzei, F., Sammartino, M. P. and Tommassetti, M., Bioelectrochemistry and Bioenergetics 23, 195 (1990).Google Scholar
7. Ho, W. O., Krause, S., McNeil, C. J., Pritchard, J. A., Armstrong, R. D., Athey, D. and Rawson, K., Analytical Chemistry 71, 1940 (1999).Google Scholar
8. Hirose, S., Hayashi, M., Tamura, N., Kamidate, T., Karube, I. and Suzuki, S., Analytica Chimica Acta 151, 377 (1983).Google Scholar
9. Osaka, T., Komaba, S., Fujino, Y., Matsuda, T. and Satoh, I., Journal of the Electrochemical Society 146(2), 615 (1999).Google Scholar
10. Singhal, R., Gambhir, A., Pandey, M. K., Annapoorni, S. and Malhotra, B. D., Biosensor and Bioelectronics 17, 697 (2002).Google Scholar
11. Mascini, M., Iannello, M. and Palleschi, G., Analytica Chimica Acta 146, 135 (1983).Google Scholar
12. Kenji, Y., Yusuke, F., Tetsuya, O. and Ikuo, S., Sensors and Actuators B 76, 152 (2001).Google Scholar
13. Kallury, K. M. R., Lee, W. E. and Thompson, M., Analytical Chemistry 64(9), 1062 (1992).Google Scholar
14. Wirde, M. and Gelius, U., Langmuir 15(19), 6370 (1999).Google Scholar