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Genetic Engineering of Polymeric Materials

Published online by Cambridge University Press:  29 November 2013

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Polymerization reactions are generally divided into two broad classes: step growth or polycondensation reactions (examples would include the synthesis of polyamides and polyesters), and chain growth processes such as those used to prepare polyethylene or polystyrene. These processes are illustrated schematically in Figure 1.

The statistical nature of step and chain growth polymerization processes ensures that the products of such reactions must be heterogeneous. Conventional polymeric materials thus consist of mixtures of chains, often characterized by relatively broad distributions of chain length or composition. In many materials applications, this kind of molecular heterogeneity is advantageous since it suppresses crystallization and helps to preserve desirable properties such as optical clarity or elasticity. On the other hand, synthetic developments that afford improved control of macromolecular architecture have had profound impact on materials science and technology. As examples, one can cite the discovery of Ziegler-Natta polymerization, now used to prepare billions of pounds per year of crystalline polyolefins, or the development of living anionic polymerization of olefins, which led directly to block copolymers and the commercially important thermoplastic elastomers.

The advent of recombinant DNA methods has provided a basis for developing polymeric materials characterized by essentially absolute uniformity of chain length, sequence, and stereochemistry. This article outlines the principles governing the cloning and expression of artificial genes, and examines the potential role of artificial proteins in polymer materials science.

Type
Polymers: Novel Techniques
Copyright
Copyright © Materials Research Society 1991

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References

1.Boor, J., Zieler-Natta Catalysts and Polymerizations (Academic Press, New York, 1979).Google Scholar
2.Szwarc, M., Adv. Polym. Sci. 49 (1983) p. 1.CrossRefGoogle Scholar
3. For typical procedures, consult Sambrook, J., Frisch, E.F., and Maniatis, T., Molecular Cloning, A Laboratory Manual, 2nd ed. (Cold Spring Harbor, 1989).Google Scholar
4.Mahajan, S.K., in Genetic Recombination, edited by Kucherlapati, R. and Smith, G.R. (American Society for Microbiology, Washington, 1988) Chapter 3.Google Scholar
5. For a brief review, see Brawerman, G., Cell 57 (1989) p. 9.CrossRefGoogle Scholar
6.Dreyfus, M., J. Mol. Biol. 204 (1988) p. 79, and references therein.CrossRefGoogle Scholar
7.Gutman, G.A. and Hatfield, G.W., Proc. Natl. Acad. Sci. U.S.A. 86 (1989) p. 3699, and references therein.CrossRefGoogle Scholar
8.Aota, S., Gojobori, T., Ishibashi, F., Maruyama, T., and Ikemura, T., Nucleic Acids Res. 16 (1988) p. r315.CrossRefGoogle Scholar
9.McGrath, K.P., Tirrell, D.A., Kawai, M., Mason, T.L., and Fournier, M.J., Biotechnol. Prog. 6 (1990) p. 188.CrossRefGoogle Scholar
10.Studier, F.W., Rosenberg, A.H., and Dunn, J.J., Methods Enzymol. 185 (1990) p. 60.CrossRefGoogle Scholar
11.Smith, D.B. and Johnson, K.S., Gene 67 (1988) p. 31.CrossRefGoogle Scholar
12.Bassett, D.C., Principles of Polymer Morphology (Cambridge University Press, Cambridge, 1981).Google Scholar
13.Fraser, R.D.B., MacRae, T.P., Stewart, F.H.C., and Suzuki, E., J. Mol. Biol. 11 (1965) p. 706.CrossRefGoogle Scholar
14.Anderson, J.M., Chen, H.H., Rippon, W.B., and Walton, A.G., J. Mol. Biol. 67 (1972) p. 459.CrossRefGoogle Scholar
15.Chou, P.Y. and Fasman, G.D., Biochemistry 13 (1974) p. 211.CrossRefGoogle Scholar
16.McGrath, K.P., Fournier, M.J., Mason, T.L., and Tirrell, D.A., submitted to J. Am. Chem. Soc.Google Scholar
17.Krejchi, M.T., Atkins, E.D.T., Fournier, M.J., Mason, T.L., and Tirrell, D.A., manuscript in preparation.Google Scholar
18.Cowie, D.B. and Cohen, G.N., Biochim. Biophys. Acta 26 (1957) p. 252.CrossRefGoogle Scholar
19.Dougherty, M.J., Kothakota, S., Fournier, M.J., Mason, T.L., and Tirrell, D.A., manuscript in preparation.Google Scholar
20.Paulmier, C., Selenium Reagents and Intermediates in Organic Synthesis (Pergamon Press, Oxford, 1986).Google Scholar
21.Zhang, G., Fournier, M.J., Mason, T.L., and Tirrell, D.A., unpublished results.Google Scholar
22.Urry, D.W., J. Protein Chem. 7 (1988) p. 1.CrossRefGoogle Scholar
23. For representative papers, see Polym. Prepr. 30 (1) (1990) p. 176ff.Google Scholar