Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-30T23:19:53.529Z Has data issue: false hasContentIssue false
  • English
  • Français

Towards a single bioactive substrate combining SERS-effect and drug release control based on thin anodic porous alumina coated with gold and with lipid bilayers

Published online by Cambridge University Press:  05 January 2017

Amirreza Shayganpour ,
Marco Salerno ,
Barbara Salis  and
Silvia Dante
Amirreza Shayganpour*
Affiliation:
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy Department of Bioengineering and Robotics, University of Genova, viale Causa 13, I-16145 Genova, Italy
Marco Salerno
Affiliation:
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy
Barbara Salis
Affiliation:
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy Department of Bioengineering and Robotics, University of Genova, viale Causa 13, I-16145 Genova, Italy
Silvia Dante
Affiliation:
Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy
*
*(Email: [email protected])
Get access

Abstract

Thin anodic porous alumina (tAPA), engineered by electrochemical anodization of aluminum and post-fabrication etching, has already shown surface-enhanced Raman scattering (SERS) activity, after overcoating with a thin gold film. On the other hand, the tAPA nanoporous surface, which is biocompatible and presents controlled roughness, has been extensively investigated as a substrate for living cell cultures. Here, we are interested in exploiting the nanoporosity of tAPA as a drug reservoir and demonstrating drug-delivery capabilities of these substrates which can be combined with the above cell-seeding and SERS activities. We focused on the loading/elution of a test drug, the nonsteroidal anti-inflammatory and analgesic molecule Diclofenac. We carried out pore loading of differently concentrated aqueous solutions of the test drug, and characterized the elution profiles by UV-Vis absorbance, using the lipid bilayers coated on the top surface as a mechanism for retarded elution from the pores, providing a more sustained release. We also demonstrated that, by changing an environmental parameter such as the pH, we can trigger an increased release of the drug. Additionally, we investigated the tAPA adsorption properties by quartz crystal microbalance technique with dissipation monitoring (QCM-D). For the purpose, anodization was carried out on an Al-coated quartz, which resulted in successful fabrication of tAPA on the sensor. Finally, the process of lipid bilayer formation on the nanoporous sensor, as well as the test drug loading, was demonstrated by QCM-D.

Keywords

nanostructureRaman spectroscopythin film
Type
Articles
Information
MRS Advances , Volume 2 , Issue 30: Nanomaterials , 2017 , pp. 1597 - 1604
Copyright
Copyright © Materials Research Society 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

§

these two authors have equally contributed to the work

References

REFERENCES

Poinern, G. E. J., Shackleton, R., Mamun, S. I., and Fawcett, D., “Significance of novel bioinorganic anodic aluminum oxide nanoscaffolds for promoting cellular response.,” Nanotechnol. Sci. Appl., vol. 4, pp. 11–24, Jan. 2011.Google Scholar
Brüggemann, D., “Nanoporous Aluminium Oxide Membranes as Cell Interfaces,” J. Nanomater., vol. 2013, pp. 1–18, 2013.Google Scholar
Sriram, G., Patil, P., Bhat, M. P., Hegde, R. M., Ajeya, K. V, Udachyan, I., Bhavya, M. B., Gatti, M. G., Uthappa, U. T., Neelgund, G. M., Jung, H., Altalhi, T., and Kurkuri, M. D., “Current Trends in Nanoporous Anodized Alumina Platforms for Biosensing Applications,” vol. 2016, 2016.Google Scholar
Toccafondi, C., La Rocca, R., Scarpellini, A., Salerno, M., Das, G., and Dante, S., “Thin nanoporous alumina-based SERS platform for single cell sensing,” Appl. Surf. Sci., vol. 351, pp. 738–745, 2015.CrossRefGoogle Scholar
[5] Losic, D. and Santos, A., Nanoporous Alumina: Fabrication, Structure, Properties and Applications. 2015.CrossRefGoogle Scholar
Nemati, M., Santos, A., Kumeria, T., and Losic, D., “Label-Free Real-Time Quantification of Enzyme Levels by Interferometric Spectroscopy Combined with Gelatin-Modified Nanoporous Anodic Alumina Photonic Films,” Anal. Chem., vol. 87, no. 17, pp. 9016–9024, 2015.CrossRefGoogle Scholar
Jeon, G., Yang, S. Y., and Kim, J. K., “Functional nanoporous membranes for drug delivery,” J. Mater. Chem., vol. 22, no. 30, p. 14814, 2012.CrossRefGoogle Scholar
Bin Liu, Z., Zhang, Y., Yu, J. J., Mak, A. F. T., Li, Y., and Yang, M., “A microfluidic chip with poly(ethylene glycol) hydrogel microarray on nanoporous alumina membrane for cell patterning and drug testing,” Sensors Actuators, B Chem., vol. 143, no. 2, pp. 776–783, 2010.Google Scholar
Simovic, S., Losic, D., and Vasilev, K., “Controlled drug release from porous materials by plasma polymer deposition.,” Chem. Commun. (Camb)., vol. 46, no. 8, pp. 1317–9, 2010.CrossRefGoogle Scholar
Aw, M. S., Simovic, S., Addai-Mensah, J., and Losic, D., “Polymeric micelles in porous and nanotubular implants as a new system for extended delivery of poorly soluble drugs,” J. Mater. Chem., vol. 21, no. 20, p. 7082, 2011.Google Scholar
Rahman, S., Ormsby, R., Santos, A., Atkins, G. J., Findlay, D. M., and Losic, D., “Nanoengineered drug-releasing aluminium wire implants: comparative investigation of nanopore geometry, drug release and osteoblast cell adhesion,” RSC Adv., vol. 5, no. 92, pp. 75004–75014, 2015.CrossRefGoogle Scholar
[12] Salerno, M., Shayganpour, A., Salis, B., and Dante, S., “Surface-Enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina,” Beilstein J. Nanotechnol., vol. in press, 2017.Google Scholar
Kozakevych, R. B., Bolbukh, Y. M., and Tertykh, V. A., “Controlled Release of Diclofenac Sodium from Silica-Chitosan Composites,” World J. Nano Sci. Eng., vol. 3, no. September, pp. 69–78, 2013.Google Scholar
Fini, A., Cavallari, C., and Ospitali, F., “Diclofenac salts. V. examples of polymorphism among diclofenac Salts with Alkyl-hydroxy Amines Studied by DSC and HSM,” Pharmaceutics, vol. 2, no. 2, pp. 136–158, 2010.Google Scholar
Iliescu, T., Baia, M., and Kiefer, W., “FT-Raman, surface-enhanced Raman spectroscopy and theoretical investigations of diclofenac sodium,” Chem. Phys., vol. 298, no. 1–3, pp. 167–174, 2004.CrossRefGoogle Scholar
Iliescu, T., Baia, M., and Maniu, D., “Raman and surface enhanced Raman spectroscopy on molecules of pharmaceutical and biological interest,” Rom. Reports Phys., vol. 60, no. 3, pp. 829–855, 2008.Google Scholar
Schulz, H. and Baranska, M., “Identification and quantification of valuable plant substances by IR and Raman spectroscopy,” Vib. Spectrosc., vol. 43, no. 1, pp. 13–25, 2007.Google Scholar
Köhler, M., MacHill, S., Salzer, R., and Krafft, C., “Characterization of lipid extracts from brain tissue and tumors using Raman spectroscopy and mass spectrometry,” Anal. Bioanal. Chem., vol. 393, no. 5, pp. 1513–1520, 2009.Google Scholar
[19] Le Ru, E. C. and Etchegoin, P. G., “A quick overview of surface-enhanced Raman spectroscopy,” in Principles of surface-enhanced Raman spectroscopy, 2009, p. 12.CrossRefGoogle Scholar
Reimhult, E., Höök, F., and Kasemo, B., “Intact vesicle adsorption and supported biomembrane formation from vesicles in solution: Influence of surface chemistry, vesicle size, temperature, and osmotic pressure,” Langmuir, vol. 19, no. 5, pp. 1681–1691, 2003.Google Scholar
Goubaidoulline, I., Vidrich, G., and Johannsmann, D., “Organic Vapor Sensing with Ionic Liquids Entrapped in Alumina Nanopores on Quartz Crystal Resonators,” Anal. Chem., vol. 77, no. 2, pp. 615–619, 2005.Google Scholar