Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-24T15:51:34.384Z Has data issue: false hasContentIssue false
  • English
  • Français

Synthetic Biology in Aqueous Compartments at the Micro- and Nanoscale

Published online by Cambridge University Press:  10 July 2017

J. Boreyko ,
P. Caveney ,
S. L. Norred ,
C. Chin ,
S.T. Retterer ,
M.L. Simpson  and
C.P. Collier
J. Boreyko
Affiliation:
Virginia Polytechnic Institute and State University, Blacksburg, VA, 24060
P. Caveney
Affiliation:
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831 Bredesen Center, University of Tennessee, Knoxville, TN 37996
S. L. Norred
Affiliation:
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831 Bredesen Center, University of Tennessee, Knoxville, TN 37996
C. Chin
Affiliation:
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831 Bredesen Center, University of Tennessee, Knoxville, TN 37996
S.T. Retterer
Affiliation:
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831 Bredesen Center, University of Tennessee, Knoxville, TN 37996 Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
M.L. Simpson
Affiliation:
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831 Bredesen Center, University of Tennessee, Knoxville, TN 37996
C.P. Collier*
Affiliation:
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831 Bredesen Center, University of Tennessee, Knoxville, TN 37996
*
*(Email: [email protected])

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Aqueous two-phase systems and related emulsion-based structures defined within micro- and nanoscale environments enable a bottom-up synthetic biological approach to mimicking the dynamic compartmentation of biomaterial that naturally occurs within cells. Model systems we have developed to aid in understanding these phenomena include on-demand generation and triggering of reversible phase transitions in ATPS confined in microscale droplets, morpho-logical changes in networks of femtoliter-volume aqueous droplet interface bilayers (DIBs) formulated in microfluidic channels, and temperature-driven phase transitions in interfacial lipid bilayer systems supported on micro and nanostructured substrates. For each of these cases, the dynamics were intimately linked to changes in the chemical potential of water, which becomes increasingly susceptible to confinement and crowding. At these length scales, where interfacial and surface areas predominate over compartment volumes, both evaporation and osmotic forces become enhanced relative to ideal dilute solutions. Consequences of confinement and crowding in cell-sized microcompartments for increasingly complex scenarios will be discussed, from single-molecule mobility measurements with fluorescence correlation spectroscopy to spatio-temporal modulation of resource sharing in cell-free gene expression bursting.

Keywords

biomaterialbiomimetic (assembly)nanostructure
Type
Articles
Information
MRS Advances , Volume 2 , Issue 45: Soft Materials and Biomaterials , 2017 , pp. 2427 - 2433
Copyright
Copyright © Materials Research Society 2017 

References

REFERENCES

Ellis, R.J., Trends Biochem. Sci. 26, 597 (2001).CrossRefGoogle Scholar
Zimmerman, S.B., and Minton, A.P., Annu. Rev. Biophys. Biomol. Struct. 22, 27 (1993).Google Scholar
Hall, D., and Minton, A.P., Biochim. Biophys. Acta 1649, 127 (2003).CrossRefGoogle Scholar
Zhou, H.-X., Rivas, G. and Minton, A.P., Annu. Rev. Biophys. 37, 375 (2008).Google Scholar
Cayley, S., and Record, M.T. Jr., J. Mol. Recognit. 17, 488 (2004).Google Scholar
Dix, J.A., and Verkman, A.S., Annu. Rev. Biophys. 37, 247 (2008).CrossRefGoogle Scholar
Mika, J.T., Poolman, B., Curr. Opin. Biotechnol. 22, 117 (2011).Google Scholar
Bénichou, O., Chevalier, C., Klafter, J., Meyer, B., and Voituriez, R., Nat. Chem. 2, 472 (2010).Google Scholar
Schuss, Z.A., Singer, D., and Holcman, D., Proc. Natl. Acad. Sci. USA 104, 16098 (2007).Google Scholar
Singh, A., and Weinberger, L.S., Curr. Opin. Microbiol. 12, 460 (2008).Google Scholar
Parsegian, V.A., Rand, R.P. and Rau, D.C., Proc. Natl. Acad. Sci. USA 97, 3987 (2000).Google Scholar
Boreyko, J.B., Mruetusatorn, P., Retterer, S.T., and Collier, C.P., Lab Chip 13, 1295 (2013).Google Scholar
Ma, S., Thiele, J., Liu, X., Bai, Y., Abell, C. and Huck, W. T. S., Small, 8, 2356 (2012).Google Scholar
Boreyko, J.B., Mruetusatorn, P., Sarles, S.A., Retterer, S.T., and Collier, C.P., J. Am. Chem. Soc. 135, 5545 (2013).Google Scholar
Mruetusatorn, P., Boreyko, J.B., Venkatesan, G., Sarles, S.A., Hayes, D., and Collier, C.P., Soft Matter 10, 2530 (2014).Google Scholar
Fowlkes, J.D. and Collier, C.P., Lab Chip 13, 877 (2013).Google Scholar
Caveney, P.M., Norred, S.E., Chin, C.W., Boreyko, J.B., Razooky, B.S., Retterer, S.T., Collier, C.P., and Simpson, M.L., ACS Synth. Biol. 6, 334 (2016).Google Scholar
So, L., Ghosh, A., Zong, C., Sepúlveda, L.A., Sergev, R., and Golding, I., Nat. Genet. 43, 554 (2011).Google Scholar
Dar, R.D., Razooky, B.S., Weinberger, L.S., Cox, C.D., and Simpson, M.L., PLoS One 10, e0140969 (2015).Google Scholar