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Polydopamine-mediated Surface Modification Promotes the Adhesion and Proliferation of Human Induced Pluripotent Stem Cells

Published online by Cambridge University Press:  04 November 2019

Yan Nie ,
Zijun Deng ,
Weiwei Wang ,
Thanga Bhuvanesh ,
Nan Ma  and
Andreas Lendlein
Yan Nie
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513, Teltow, Germany Institute of Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany
Zijun Deng
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513, Teltow, Germany Institute of Chemistry and Biochemistry, Free University of Berlin, 14195, Berlin, Germany
Weiwei Wang
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513, Teltow, Germany
Thanga Bhuvanesh
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513, Teltow, Germany Institute of Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany
Nan Ma*
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513, Teltow, Germany Institute of Chemistry and Biochemistry, Free University of Berlin, 14195, Berlin, Germany Helmholtz Virtual Institute − Multifunctional Materials in Medicine, Berlin and Teltow, Germany
Andreas Lendlein*
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513, Teltow, Germany Institute of Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany Institute of Chemistry and Biochemistry, Free University of Berlin, 14195, Berlin, Germany Helmholtz Virtual Institute − Multifunctional Materials in Medicine, Berlin and Teltow, Germany
*
*To whom correspondence should be addressed: Prof. Dr. Nan Ma, email: [email protected] Prof. Dr. Andreas Lendlein, email: [email protected]
*To whom correspondence should be addressed: Prof. Dr. Nan Ma, email: [email protected] Prof. Dr. Andreas Lendlein, email: [email protected]
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Abstract

With their abilities of self-renewal and pluripotency to differentiate into all three germ layers, human induced pluripotent stem cells (hiPSCs) are a promising cell source for cell-based drug and implant testing. However, the large-scale expansion and maintenance of hiPSCs requires following strict protocols. There is high demand for advanced cell culture systems capable of generating high-quality hiPSCs to meet application requirements. In this study, we probe the possibility of modifying polymeric substrates for maintaining the self-renewal and pluripotency of hiPSCs. Here, polydopamine (PDA) was employed to immobilize the Laminin 521 (LN521) onto the surface of polyethylene terephthalate (PET). An aqueous solution of dopamine with concentrations ranging from 0 to 2.0 mg/mL was applied on PET surfaces. These PDA-modified surfaces were further functionalized with LN521. Surface wettability was evaluated by measuring the water contact angle (WCA) and surface properties of the modified substrate were analyzed using an atomic force microscope (AFM). Initial hiPSC attachment (1h after seeding) and cell proliferation were evaluated by counting the total cell number. The maintenance of pluripotency was evaluated at designed time points. WCA of the PDA-LN521 surfaces gradually decreased from 62.1°±6.3° to 8.1°±2.9°. The maximum peak-to-valley height roughness (Rt) of those surfaces determined by AFM increased in a dopamine-concentration-dependent manner, ranging from 43.9±1.6 nm to 126.7±7.6 nm. The Young’s modulus of these surfaces was substantially increased from 0.98±0.36 GPa to 4.81±2.41 GPa. There was a significant enhancement (13.0±7.2% and 24.2±8.1%) of hiPSC adhesion on PDA-LN521 (dopamine concentration at 0.125 and 0.25 mg/mL). When increasing the dopamine concentration to 0.5 and 1.0 mg/mL, there was no further increase in hiPSC adhesion on PDA-LN521 surfaces. Moreover, hiPSC proliferation was remarkably enhanced on PDA-LN521 surface (dopamine solution at concentration from 0.125 to 1.0 mg/mL). Pluripotency of hiPSCs was not affected by PDA treatment. In conclusion, PDA-mediated surface modification is an effective approach for the robust expansion and maintenance of hiPSCs on polymer substrates.

Keywords

biomaterialbiomedicalcoatingpolymerbiological
Type
Articles
Information
MRS Advances , Volume 5 , Issue 12-13: Soft Materials and Biomaterials , 2020 , pp. 591 - 599
Copyright
Copyright © Materials Research Society 2019

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