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Physical characterization and in vitro evaluation of 3D printed hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON structures made by lithographic ceramic manufacturing

Published online by Cambridge University Press:  29 April 2020

Alexander K. Nguyen Open the ORCID record for Alexander K. Nguyen [Opens in a new window] ,
Peter L. Goering ,
Shelby A. Skoog  and
Roger J. Narayan
Alexander K. Nguyen
Affiliation:
Joint UNC/NCSU Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina, United States Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD
Peter L. Goering
Affiliation:
Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD
Shelby A. Skoog
Affiliation:
Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD
Roger J. Narayan*
Affiliation:
Joint UNC/NCSU Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina, United States
*
*(Email: [email protected])
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Abstract

In this study, lithographic ceramic manufacturing was used to create solid chips out of hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON ceramic. X-ray powder diffraction of each material confirmed that the chips were crystalline, with little amorphous character that could result from remaining polymeric binder, and were composed entirely out of the ceramic feedstock. Surface morphologies and roughnesses were characterized using atomic force microscopy. Human bone marrow stem cells cultured with osteogenic supplements on each material type expressed alkaline phosphatase levels, an early marker of osteogenic differentiation, on par with cells cultured on a glass control. However, cells cultured on the tricalcium phosphate-containing material expressed lower levels of ALP suggesting that osteoinduction was impaired on this material. Further analyses should be conducted with these materials to identify underlying issues of the combination of material and analysis method.

Keywords

biomaterialadditive manufacturing
Type
Articles
Information
MRS Advances , Volume 5 , Issue 46-47: Soft Materials and Biomaterials , 2020 , pp. 2419 - 2428
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Copyright
Copyright © Materials Research Society 2020

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