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Functionalization and Characterization of an MRI-Capable, Targeted Nanoparticle Platform for Delivery to the Brain

Published online by Cambridge University Press:  10 April 2018

S. M. Curley ,
J. Castracane ,
M. Bergkvist  and
N. C. Cady
S. M. Curley*
Affiliation:
SUNY Polytechnic Institute, Colleges of Nanoscale Sci. & Eng. Albany, NY
J. Castracane
Affiliation:
SUNY Polytechnic Institute, Colleges of Nanoscale Sci. & Eng. Albany, NY
M. Bergkvist
Affiliation:
SUNY Polytechnic Institute, Colleges of Nanoscale Sci. & Eng. Albany, NY
N. C. Cady
Affiliation:
SUNY Polytechnic Institute, Colleges of Nanoscale Sci. & Eng. Albany, NY
*
*(Email: [email protected])
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Abstract

Novel methods are needed to traverse the blood-brain barrier and deliver drugs to specific targets in the brain. To this end, MS2 bacteriophage was explored as a multifunctional transport and targeting vector. The MS2 capsid exterior was modified with two different targeting moieties for delivery across the BBB and targeting specific regions of interest in the brain. Successful modification of MS2 capsids with a brain targeting peptide and NMADAR2D-targeting antibody was confirmed by immunoblotting and fluorescence detection. To measure transport efficiency of MS2 particles across an in vitro BBB model, a highly sensitive RT-qPCR protocol was developed and implemented. Finally, in order to demonstrate the potential of MS2 as a drug delivery vehicle, nucleotide-mediated loading of capsids was investigated with the MRI contrast agent Gd-DOTA modified with psoralen.

Keywords

biomaterialbiomedicalnanoscalesurface chemistry
Type
Articles
Information
MRS Advances , Volume 3 , Issue 50: Soft Materials and Biomaterials , 2018 , pp. 3027 - 3032
Copyright
Copyright © Materials Research Society 2018 

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References

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