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Freeze-cast Porous Chitosan Conduit for Peripheral Nerve Repair

Published online by Cambridge University Press:  20 February 2018

Kaiyang Yin ,
Prajan Divakar ,
Jennifer Hong ,
Karen L. Moodie ,
Joseph M. Rosen ,
Cathryn A. Sundback ,
Michael K. Matthew  and
Ulrike G.K. Wegst
Kaiyang Yin
Affiliation:
Thayer School of Engineering, Dartmouth College, Hanover, NH03755, U.S.A.
Prajan Divakar
Affiliation:
Thayer School of Engineering, Dartmouth College, Hanover, NH03755, U.S.A.
Jennifer Hong
Affiliation:
Dartmouth-Hitchcock Medical Center, Lebanon, NH03756, U.S.A.
Karen L. Moodie
Affiliation:
Geisel School of Medicine, Dartmouth College, Hanover, NH0375, U.S.A. Dartmouth-Hitchcock Medical Center, Lebanon, NH03756, U.S.A.
Joseph M. Rosen
Affiliation:
Thayer School of Engineering, Dartmouth College, Hanover, NH03755, U.S.A. Geisel School of Medicine, Dartmouth College, Hanover, NH0375, U.S.A. Dartmouth-Hitchcock Medical Center, Lebanon, NH03756, U.S.A.
Cathryn A. Sundback
Affiliation:
Center for Regenerative Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA02114, U.S.A.
Michael K. Matthew
Affiliation:
Geisel School of Medicine, Dartmouth College, Hanover, NH0375, U.S.A. Dartmouth-Hitchcock Medical Center, Lebanon, NH03756, U.S.A.
Ulrike G.K. Wegst*
Affiliation:
Thayer School of Engineering, Dartmouth College, Hanover, NH03755, U.S.A.
*
*(Email: [email protected], [email protected])
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Abstract

A novel freeze-cast porous chitosan conduit for peripheral nerve repair with highly-aligned, double layered porosity, which provides the ideal mechanical and chemical properties was designed, manufactured, and assessed in vivo. Efficacies of the conduit and the control inverted nerve autograft were evaluated in bridging 10-mm Lewis rat sciatic nerve gap at 12 weeks post-implantation. Biocompatibility and regenerative efficacy of the porous chitosan conduit were evaluated through the histomorphometric analysis of longitudinal and transverse sections. The porous chitosan conduit was found to have promising regenerative characteristics, promoting the desired neovascularization, and axonal ingrowth and alignment through a combination of structural, mechanical and chemical cues.

Keywords

BiomaterialsDirectional solidificationPolymers
Type
Articles
Information
MRS Advances , Volume 3 , Issue 30: Biomaterials and Soft Materials , 2018 , pp. 1677 - 1683
Copyright
Copyright © Materials Research Society 2018 

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References

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