Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-28T13:30:21.032Z Has data issue: false hasContentIssue false
  • English
  • Français

Upconversion Luminescence Imaging of Tumors with EGFR-Affibody Conjugated Nanophosphors

Published online by Cambridge University Press:  20 May 2019

Majid Badieirostami ,
Colin Carpenter ,
Guillem Pratx ,
Lei Xing  and
Conroy Sun Open the ORCID record for Conroy Sun [Opens in a new window]
Majid Badieirostami
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Colin Carpenter
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Guillem Pratx
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Lei Xing
Affiliation:
Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, CA94305
Conroy Sun*
Affiliation:
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Portland, OR97201 Department of Radiation Medicine, School of Medicine, Oregon Health & Science University, Portland, Oregon97239
*
*(Email: [email protected])
Get access

Abstract

Near infrared (NIR) optical imaging has demonstrated significant potential as an effective modality for cancer molecular imaging. Among various NIR probes currently under investigation, upconversion nanophosphors (UCNPs) possess great promise due to their anti-Stokes emission and sequential photon absorption which result in superior detection sensitivity and a simple imaging setup, respectively. Here we investigated the utility of this imaging modality to detect tumor cells expressing the epidermal growth factor receptor (EGFR) using affibody functionalized nanophosphors and a custom built imaging system. Initially, aqueous dispersible NaYF4: Tm+3, Yb+3 UCNPs were synthesized and their photophysical properties were characterized. Then, their luminescence response as a function of concentration and their depth resolving capability in a tissue-simulating phantom were examined. Finally, we demonstrated the use of bioconjugated UCNPs for imaging EGFR-expressing tumors both in vitro and in vivo. Our data suggests that NIR imaging with UCNPs may be useful for noninvasive imaging of tumors.

Keywords

Lanthanidenanoscalebiomaterial
Type
Articles
Information
MRS Advances , Volume 4 , Issue 46-47: Soft Materials and Biomaterials , 2019 , pp. 2461 - 2470
Copyright
Copyright © Materials Research Society 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Massoud, T. F. and Gambhir, S. S., “Molecular imaging in living subjects: seeing fundamental biological processes in a new light,” Genes & Development 17, 545-580 (2003).CrossRefGoogle Scholar
Weissleder, R., “Molecular imaging in cancer,” Science 312, 1168-1171 (2006).CrossRefGoogle Scholar
Weissleder, R. and Pittet, M. J., “Imaging in the era of molecular oncology,” Nature 452, 580-589 (2008).CrossRefGoogle ScholarPubMed
Hilderbrand, S. A. and Weissleder, R., “Near-infrared fluorescence: application to in vivo molecular imaging,” Current Opinion in Chemical Biology 14, 71–79 (2010).CrossRefGoogle ScholarPubMed
He, X., Gao, J., Gambhir, S. S., and Cheng, Z., “Near-infrared fluorescent nanoprobes for cancer molecular imaging: status and challenges,” Trends in Molecular Medicine 16, 574-583 (2010).CrossRefGoogle ScholarPubMed
Ke, S., Wen, X., Gurfinkel, M., Charnsangavej, C., Wallace, S., Sevick-Muraca, E. M., and Li, C., “Near-Infrared Optical Imaging of Epidermal Growth Factor Receptor in Breast Cancer Xenografts,” Cancer Research 63, 7870-7875 (2003).Google ScholarPubMed
Keereweer, S., Kerrebijn, J. D. F., Mol, I. M., Mieog, J. S. D., Van Driel, P. B. A. A., de Jong, R. J. B., Vahrmeijer, A. L., and Lowik, C. W. G. M., “Optical imaging of oral squamous cell carcinoma and cervical lymph node metastasis,” Head & Neck 34, 1002-1008 (2012).CrossRefGoogle ScholarPubMed
Chatterjee, D. K., Rufaihah, A. J., Zhang, Y., “Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals,” Biomaterials 29, 937-943 (2008).CrossRefGoogle ScholarPubMed
Hilderbrand, S. A., Shao, F., Salthouse, C., Mahmood, U., and Weissleder, R., “Upconverting luminescent nanomaterials: application to in vivo bioimaging,” Chemical Communications, 4188-4190 (2009).CrossRefGoogle ScholarPubMed
Kobayashi, H., Kosaka, N., Ogawa, M., Morgan, N. Y., Smith, P. D., Murray, C. B., Ye, X., Collins, J., Kumar, G. A., Bell, H., and Choyke, P. L., “In vivo multiple color lymphatic imaging using upconverting nanocrystals,” Journal of Materials Chemistry 19, 6481–6484 (2009).CrossRefGoogle Scholar
Modjahedi, H., and Dean, C., “The receptor for EGF and its ligands: expression, prognostic value and target for therapy in cancer,” International Journal of Oncology 4, 277–296 (1994).Google Scholar
Costa, S., Stamm, H., Almendral, A., Ludwig, H., Wyss, R., Fabbro, D., Ernst, A., Takahashi, A., and Eppenberger, U., “Predictive value of EGF receptor in breast cancer,” Lancet 2, 1258, 1988.CrossRefGoogle ScholarPubMed
Ang, K. K., Berkey, B. A., Tu, X., Zhang, H. Z., Katz, R., Hammond, E. H., Fu, K. K., and Milas, L., “Impact of epidermal growth factor receptor expression on survival and pattern of relapse in patients with advanced head and neck carcinoma,” Cancer Research 62, 7350–7356 (2002).Google ScholarPubMed
Salthouse, C., Hildebrand, S., Weissleder, R., and Mahmood, U., “Design and demonstration of a small-animal upconversion imager,” Optics Express 16, 21731-21737 (2008).CrossRefGoogle Scholar
Boyer, J. C., Vetrone, F., Cuccia, L. A., and Capobianco, J. A., “Synthesis of colloidal upconverting NaYF4 nanocrystals doped with Er3+, Yb3+ and Tm3+, Yb3+ via thermal decomposition of lanthanide trifluoroacetate precursors,” Journal of American Chemical Society 128, 7444-7445 (2006).CrossRefGoogle ScholarPubMed
De Grand, A. M., Lomnes, S. J., Lee, D. S., Pietrzykowski, M., Ohnishi, S., Morgan, T. G., Gogbashian, A., Laurence, R. G., and Frangioni, J. V., “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” Journal of Biomedical Optics 11, 014007-1-10 (2006).CrossRefGoogle ScholarPubMed
Pleijhuis, R. G., Langhout, G. C., Helfrich, W., Themelis, G., Sarantopoulos, A., Crane, L. M. A., Harlaar, N. J., de Jong, J. S., Ntziachristos, V., van Dam, G. M., “Near-infrared fluorescence (NIRF) imaging in breast-conserving surgery: Assessing intraoperative techniques in tissue-simulating breast phantoms,” European Journal of Surgical Oncology 37, 32-39 (2011).CrossRefGoogle ScholarPubMed
Vinegoni, C., Razansky, D., Hilderbrand, S. A., Shao, F., Ntziachristos, V., and Weissleder, R., “Transillumination fluorescence imaging in mice using biocompatible upconverting nanoparticles,” Optics Letters 34, 2566-2568 (2009).CrossRefGoogle ScholarPubMed
Milas, L., Fan, Z., Andratschke, N. H., and Ang, K. K., “Epidermal growth factor receptor and tumor response to radiation: in vivo preclinical studies,” International Journal of Radiation Oncology Biology Physics 58, 966-971 (2004).CrossRefGoogle ScholarPubMed
Yang, L., Mao, H., Wang, Y. A., Cao, Z., Peng, X., Wang, X., Duan, H., Ni, C., Yuan, Q., Adams, G., Smith, M. Q., Wood, W. C., Gao, X., and Nie, S. “Single chain epidermal growth factor receptor antibody conjugated nanoparticles for in vivo tumor targeting and imaging,” Small 5, 235-243 (2009).CrossRefGoogle ScholarPubMed