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Indirect versus direct 3D printing of hydrogel scaffolds for adipose tissue regeneration

Published online by Cambridge University Press:  17 February 2020

Lana Van Damme ,
Emilie Briant ,
Phillip Blondeel  and
Sandra Van Vlierberghe
Lana Van Damme
Affiliation:
Polymer Chemistry & Biomaterials Group – Centre of Macromolecular Chemistry (CMaC) – Gent Alliance for Tissue Engineering (GATE) – Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281, S4-Bis, 9000 Ghent, Belgium
Emilie Briant
Affiliation:
Polymer Chemistry & Biomaterials Group – Centre of Macromolecular Chemistry (CMaC) – Gent Alliance for Tissue Engineering (GATE) – Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281, S4-Bis, 9000 Ghent, Belgium
Phillip Blondeel
Affiliation:
Department of Plastic & Reconstructive Surgery, Ghent University Hospital, Corneel Heymanslaan 10, 2K12, 9000 Ghent, Belgium
Sandra Van Vlierberghe*
Affiliation:
Polymer Chemistry & Biomaterials Group – Centre of Macromolecular Chemistry (CMaC) – Gent Alliance for Tissue Engineering (GATE) – Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281, S4-Bis, 9000 Ghent, Belgium
*
*Corresponding author: Prof. Sandra Van Vlierberghe [email protected]
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Abstract

There exists a need for an innovative reconstructive approach for breast reconstruction, tackling current drawbacks and limitations present in the clinic. In this respect, adipose tissue engineering could offer a promising alternative. We have previously shown that methacrylamide-functionalized gelatin scaffolds are suitable to support the adhesion of adipose tissue-derived stem cells as well as their subsequent differentiation into the adipogenic lineage. The current paper aims to compare different techniques to produce such scaffolds including direct versus indirect 3D printing. Extrusion-based (direct) 3D printing was compared to indirect 3D printing exploiting a polylactic acid (PLA) sacrificial mould, thereby focussing on the physico-chemical characteristics of the obtained scaffolds. The results indicate that similar properties can be achieved irrespective of the technique applied. It can therefore be concluded that indirect 3D printing could offer some benefits over direct additive manufacturing (AM) as a more complex design can be created while materials that were previously unsuited for direct printing because of limitations associated with their characteristics (e.g. low viscosity), could potentially be applied as starting materials for indirect 3D printing to generate porous constructs with full control over their design.

Keywords

3D printingbiomaterial
Type
Articles
Information
MRS Advances , Volume 5 , Issue 17: Soft Materials and Biomaterials , 2020 , pp. 855 - 864
Copyright
Copyright © Materials Research Society 2020

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