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The Effect of Graphene Oxide/Reduced Graphene Oxide Functionalized with Metal Nanoparticles on Dermal, Bacterial, and Cancerous/Non-Cancerous Epidermal Cells

Published online by Cambridge University Press:  12 January 2016

Rebecca Isseroff ,
Arthur Chen ,
Jae Cho ,
Marcia Simon ,
Luckner J. Jerome  and
Miriam Rafailovich
Rebecca Isseroff*
Affiliation:
Lawrence High School, Cedarhurst, NY, USA. Dept. of Materials Science and Engineering, SUNY Stony Brook, Stony Brook, NY, USA.
Arthur Chen
Affiliation:
Lawrence High School, Cedarhurst, NY, USA.
Jae Cho
Affiliation:
Boston University, Boston, MA, USA.
Marcia Simon
Affiliation:
Dept. of Materials Science and Engineering, SUNY Stony Brook, Stony Brook, NY, USA.
Luckner J. Jerome
Affiliation:
Suffolk County Community College, Selden, NY, USA
Miriam Rafailovich
Affiliation:
Dept. of Materials Science and Engineering, SUNY Stony Brook, Stony Brook, NY, USA.
*
*(Email: [email protected])
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Abstract

The unique planar structures and high surface area of graphene oxide (GO) and reduced graphene oxide (rGO) has induced great interest as drug delivery platforms. Silver and platinum nanoparticles are used in medicine, biotechnology and cosmetics and have electrocatalytic properties. However, GO has been found to be toxic to a variety of cells; pure graphene is insoluble; and nanoparticles aggregate, diminishing their activity. This research functionalized GO and rGO with silver or platinum nanoparticles (Ag/PtNPs) and experimental solutions were then tested on bacteria, dermal fibroblasts (DFBC’s), and cancerous (SCC13’s) and non-cancerous (DO33’s) epidermal cells to determine toxicity and/or cell viability.

GO was functionalized with Ag or Pt salts, forming metalized-GO; then reduced with NaBH4. Ag-rGO, depending on nanoparticle size, killed either S. Aureus or K. Pneumoniae, while Pt-rGO and rGO had no effect. 1mM Ag-prGO concentrations diluted 1:100 with DMEM was toxic to SCC13 cancerous keratinocytes but showed reduced toxicity to DO33 non-cancerous keratinocytes; whereas Pt-rGO and rGO exerted little effect on SCC13’s and DO33’s at all concentrations. All test solutions adhered to DFBC’s and influenced the orientation of their growth, suggesting potential use in wound dressings to aid healing.

Keywords

nanostructureAgbiomaterial
Type
Articles
Information
MRS Advances , Volume 1 , Issue 22: Electronics and Photonics , 2016 , pp. 1583 - 1590
Copyright
Copyright © Materials Research Society 2016 

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References

REFERENCES

Wang, Y., Li, Z., Wang, J., Li, J., & Lin, Y. (2011). Graphene and graphene oxide: Biofunctionalization and applications in biotechnology. Trends in Biotechnology, 29(5), 205–212.Google Scholar
Feng, L., Wu, L., & Qu, X. (2012). New Horizons for Diagnostics and Therapeutic Applications of Graphene and Graphene Oxide. Adv. Mater. Advanced Materials, 168–186.Google ScholarPubMed
Shen, H., Zhang, L., Liu, M., & Zhang, Z. (n.d.). Biomedical Applications of Graphene. Theranostics, 283–294.CrossRefGoogle Scholar
Liu, J., Cui, L., & Losic, D. (2013). Graphene and graphene oxide as new nanocarriers for drug delivery applications. Acta Biomaterialia,9(12), 9243–9257.Google Scholar
Arora, S., Tyagi, N., Bhardwaj, A., Rusu, L., Palanki, R., Vig, K.,… Singh, S. (n.d.). Silver nanoparticles protect human keratinocytes against UVB radiation-induced DNA damage and apoptosis: Potential for prevention of skin carcinogenesis. Nanomedicine: Nanotechnology, Biology and Medicine, 1265–1275.Google Scholar
Jeong, SH, Yeo, SY, Yi, SC: The effect of filler particle size on the antibacterial properties of compounded polymer/silver fibers. J Mater Sci 2005, 40:5407–5411.Google Scholar
Ali-Boucetta, H., Bitounis, D., Raveendran-Nair, R., Servant, A., Bossche, J., & Kostarelos, K. (2012). Purified Graphene Oxide Dispersions Lack In Vitro Cytotoxicity and In Vivo Pathogenicity. Advanced Healthcare Materials, 433–441.Google Scholar
Liao, KH 1, Lin, YS, Macosko, CW, Haynes CL Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. ACS Appl Mater Interfaces. 2011 Jul;3(7):2607–15.CrossRefGoogle Scholar
Tobias, Lammel, Paul, Boisseaux, Maria-Luisa, Fernández-Cruz and José, M Navas Internalization and cytotoxicity of graphene oxide and carboxyl graphene nanoplatelets in the human hepatocellular carcinoma cell line Hep G2 Particle and Fibre Toxicology (2013) 10:27 Google Scholar
Singh, S., Singh, M., Kulkarni, P., Sonkar, V., Grácio, J., & Dash, D. (2012). Amine-Modified Graphene: Thrombo-Protective Safer Alternative to Graphene Oxide for Biomedical Applications. ACS Nano, 2731–2740.Google Scholar
Jassby, D. (2011). Impact of Particle Aggregation on Nanoparticle Reactivity. Duke Dissertations.Google Scholar
Gambinossi, F., Mylon, S., & Ferri, J. (2015). Aggregation kinetics and colloidal stability of functionalized nanoparticles. Advances in Colloid and Interface Science, 332–349.CrossRefGoogle ScholarPubMed
Liu, HL, Dai, SA, Fu, KY, Hsu, SH (2010). Antibacterial properties of silver nanoparticles in three different sizes and their nanocomposites with a new waterborne polyurethane. Int J Nanomedicine. 2010 Nov 19;5:1017–28.Google Scholar
Shaobin, Liu, Tingying, Helen Zeng, Mario, Hofmann, Ehdi, Burcombe, Jun, Wei⊥, Rongrong, Jiang, Jing, Kong, and Yuan, Chen. Antibacterial Activity of Graphite, Graphite Oxide, Graphene Oxide, and Reduced Graphene Oxide: Membrane and Oxidative Stress, ACS Nano, 2011, 5 (9), pp 6971–6980 Google Scholar
Hummers, W., & Offeman, R. (n.d.). Preparation of Graphitic Oxide. J. Am. Chem. Soc. Journal of the American Chemical Society, 1339–1339.CrossRefGoogle Scholar
Dulbecco, R. (1954). Plaque Formation and Isolation Of Pure Lines With Poliomyelitis Viruses. Journal of Experimental Medicine, 167–182.Google Scholar