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Mineralogy of Pb-P grains in the roots of Agrostis capillaris L. by ATEM and EXAFS*

Published online by Cambridge University Press:  05 July 2018

J. D. Cotter-Howells ,
P. E. Champness  and
J. M. Charnock
J. D. Cotter-Howells
Affiliation:
Department of Earth Sciences, The University of Manchester, Manchester M13 9PL, UK School of Biological Sciences, The University of Manchester, Manchester M13 9PL, UK
P. E. Champness
Affiliation:
Department of Earth Sciences, The University of Manchester, Manchester M13 9PL, UK
J. M. Charnock
Affiliation:
Department of Earth Sciences, The University of Manchester, Manchester M13 9PL, UK CLRC Daresbury Laboratory, Warrington WA4 4AD, UK
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Abstract

Analytical transmission electron microscopy (ATEM) and X-ray absorption spectroscopy (XAS) have been used to determine the mineralogy of Pb-P deposits in the roots of the heavy metal tolerant grass cultivar Agrostis capillaris L. cv. Parys Mountain. The deposits have a pyromorphite (Pb5(PO4)3Cl)-type structure and composition although some of the Cl may be substituted by OH. Energy-dispersive mapping under the scanning electron microscope demonstrated that the majority of these deposits are present in the outer cell wall of the epidermis (the outermost layer of root cells). The phosphate composition of these grains contrasts with the phytate (C6H18O24P612−) composition of Zn-P deposits observed in similar electron microscopy studies. The physiological role of heavy metal P deposits is unclear. Heavy metal P precipitates may form actively as a tolerance mechanism to heavy metals or passively, sequestering P in a metabolically inactive form.

Keywords

leadlead phosphatepyromorphitelead hydroxyapatiteelectron microscopyelectron diffractionheavy metal toleranceAgrostis capillaris
Type
Research Article
Information
Mineralogical Magazine , Volume 63 , Issue 6 , December 1999 , pp. 777 - 789
Copyright
Copyright © The Mineralogical Society of Great Britain and Ireland 1999

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Footnotes

†

Present address: Department of Plant and Soil Science, Cruickshank Building, Aberdeen University, Aberdeen AB24 3UU, UK.

*

The first four papers in this issue were presented at the Mineralogical Society's Spring Meeting, held on 18th March 1997, entitled ‘Phosphates: biogenic to exotic’

References

Allen, S.E. (1989) Chemical Analysis of Ecological Materials. Blackwell Scientific, Oxford.Google Scholar
Binsted, N., Campbell, J.W., Gurman, S.J. and Stephenson, P.C. (1991) SERC Daresbury Laboratory EXCURV92 Program. Daresbury Laboratory, Warrington, UK.Google Scholar
Binsted, N, Strange, R.W. and Hasnain, S.S. (1992) Constrained and restrained refinement in EXAFS data analysis with curved wave theory. Biochem., 31, 12117–25.CrossRefGoogle ScholarPubMed
Brückner, S., Lusvardi, G., Menabue, L. and Saladini, M. (1995) Crystal structure of lead hydroxyapatite from powder X-ray diffraction data. Inorg. Chim. Acta, 236, 209–12.CrossRefGoogle Scholar
Charnock, J.M. (1995) Biological applications of EXAFS spectroscopy. Radiat. Phys. Chem., 45, 385–91.CrossRefGoogle Scholar
Clark, A.M. (1993) Hey's Mineral Index: Mineral Species, Varieties and Synonyms. (3rd edition). Chapman & Hall, London.Google Scholar
Cotter-Howells, J.D. (1996) Lead phosphate formation in soils. Environ. Poll'., 93, 9–16.CrossRefGoogle ScholarPubMed
Cotter-Howells, J.D. and Caporn, S.J. (1996) Remediation of contaminated land by formation of heavy metal phosphates. Appl. Geochem., 11, 335–42.CrossRefGoogle Scholar
Cotter-Howells, J.D., Champness, P.E., Charnock, J.M. and Pattrick, R.A.D. (1994) Identification of pyromorphite in mine-waste contaminated soils by ATEM and EXAFS. Eur. J. Soil Sci., 45, 393–402.CrossRefGoogle Scholar
Dai, Y. and Hughes, J.M. (1989) Crystal structure refinements of vanadinite and pyromorphite. Canad. Mineral., 27, 189–92.Google Scholar
Davis, A., Drexler, J.W., Ruby, M.V. and Nicholson, A. (1993) Micromineralogy of mine wastes in relation to lead bioavailability, Butte. Environ. Sci. Technol., 27, 1415–25.CrossRefGoogle Scholar
Krämer, U., Cotter-Howells, J.D., Charnock, J.M., Baker, A.J.M. and Smith, J.A.C. (1996) Free histidine as a metal chelator in plants that accumulate nickel. Nature, 379, 635–8.CrossRefGoogle Scholar
Ma, Q.Y., Logan, T.J. and Traina, S.J. (1995) Lead immobilisation from aqueous solutions and contaminated soils using phosphate rocks. Environ. Sci. Technol., 29, 1118–26.CrossRefGoogle ScholarPubMed
Malone, C. Koeppe, D.E. and Miller, R.J. (1974) Localisation of lead accumulated by corn plants. Plant Physiol., 53, 388-94.CrossRefGoogle Scholar
Marschner, H. (1995) Mineral Nutrition of Higher Plants. (2nd edition). Academic Press, London.Google Scholar
Nriagu, J.O (1974) Lead orthophosphates-IV. Formation and stability of chloropyromorphite at 25°C. Geochim. Cosmochim. Acta, 37, 367–77.CrossRefGoogle Scholar
Nriagu, J.O. (1984) Folrnation and stability of base metal phosphates in soils and sediments. In Phosphate Minerals (Nriagu, J.O. and Moore, P.B., eds). Springer-Verlag, London, pp. 318–29.CrossRefGoogle Scholar
Nriagu, J.O. and Moore, P.B., Eds (1984) Phosphate Minerals. Springer-Verlag, London CrossRefGoogle Scholar
Posthuma, L. and Straalen, N.M.V. (1993) Heavy-metal adaptation in terrestrial invertebrates: a review of occurrence, genetics, physiology and ecological consequences. Comp. Biochem. Physiol, 106C, 11–38.Google Scholar
Rauser, W.E. (1990) Phytochelatins. Ann. Rev. Biochem., 59, 61–86.CrossRefGoogle ScholarPubMed
Ruby, M.V., Davis, A. and Nicholson, A. (1994) In situ formation of lead phosphates in soils as a method to immobilize lead. Environ. Sci. Technol., 28, 646–54.CrossRefGoogle ScholarPubMed
Simkiss, K. and Taylor, M.G. (1989) Convergence of cellular systems of metal detoxification. Marine Environ. Res., 28, 211–14CrossRefGoogle Scholar
Turnau, K., Kottke, I. and Oberwinkler, F. (1993) Element localisation in mycorrhizal roots of Pteridium aquilinum (L.) Kuhn collected from experimental plots treated with cadmium dust. New Phytol., 123, 313–24.CrossRefGoogle Scholar
van Steveninck, R.F.M., van Stevenlnck, M.E., Femando, D.R., Godbold, D.L., Horst, .W.J. and Marschner, H. (1987 a) Identification of zinc containing globules in roots of a zinc tolerant ecotype of Deschampsia cespitosa . J. Plant Nutr., 10, 1239–46.CrossRefGoogle Scholar
van Steveninck, R.F.M., van Steveninck, M.E., Fernando, D.R, Horst, W.J. and Marschner, H. (1987 b) Deposition of zinc phytate in globular bodies in roots of Deschampsia cespitosa ecotypes; a detoxification mechanism? J. Plant Physiol., 131, 247 –57.Google Scholar
van Steveninck, R.F.M., van Steveninck, M.E., Wells, AJ. and Femando, D.R. (1990) Zinc tolerance and the binding of zinc as zinc phytate in Lemna minor. X-ray microanalytical evidence. J. Plant Physiol., 137, 140–6.CrossRefGoogle Scholar
Vázquez, M.D., Poshenrieder, Ch., Barceló, J., Baker, A.J.M., Hatton, P. and Cope, G.H. (1994) Compartmentation of zinc in roots and leaves of the zinc hyperaecumulator Thlaspi caerulescens J. and C. Presl. Bot. Acta, 107, 243–50.CrossRefGoogle Scholar