Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T12:11:56.386Z Has data issue: false hasContentIssue false
  • English
  • Français

Vibrio cholerae O1 found attached to the dinoflagellate Noctiluca scintillans in Argentine shelf waters

Published online by Cambridge University Press:  06 January 2011

Rut Akselman ,
Verónica Jurquiza ,
Marcela C. Costagliola ,
Sol González Fraga ,
Mariana Pichel ,
Constanza Hozbor ,
Silvia Peressutti  and
Norma Binsztein
Rut Akselman*
Affiliation:
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), C.C. 175, 7600—Mar del Plata, Argentina
Verónica Jurquiza
Affiliation:
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), C.C. 175, 7600—Mar del Plata, Argentina
Marcela C. Costagliola
Affiliation:
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), C.C. 175, 7600—Mar del Plata, Argentina
Sol González Fraga
Affiliation:
Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas (ANLIS) ‘C.G. Malbrán’, Avenida V. Sarsfield 563, Buenos Aires, Argentina
Mariana Pichel
Affiliation:
Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas (ANLIS) ‘C.G. Malbrán’, Avenida V. Sarsfield 563, Buenos Aires, Argentina
Constanza Hozbor
Affiliation:
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), C.C. 175, 7600—Mar del Plata, Argentina
Silvia Peressutti
Affiliation:
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), C.C. 175, 7600—Mar del Plata, Argentina
Norma Binsztein
Affiliation:
Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas (ANLIS) ‘C.G. Malbrán’, Avenida V. Sarsfield 563, Buenos Aires, Argentina
*
Correspondence should be addressed to: R. Akselman, Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), C.C. 175, 7600—Mar del Plata, Argentina email: [email protected]
Get access

Abstract

We found viable non-culturable (VNC) Vibrio cholerae O1 attached to the cell envelope of the dinoflagellate Noctiluca scintillans. The significance of this find lies in the fact that N. scintillans could be a reservoir organism of VNC forms of V. cholerae in inter-epidemic periods, particularly when the extensive geographical distribution and the frequently high abundance of this dinoflagellate in marine environments is taken into account.

Keywords

Vibrio choleraeNoctiluca scintillansArgentinacholeraplanktonenvironment
Type
Research Article
Information
Marine Biodiversity Records , Volume 3 , June 2010 , e120
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Akselman, R., Benavides, H.R., Negri, R.M. and Carreto, J.I. (1986) Observaciones sobre especies causantes de discoloraciones en el Mar Argentino. Physis (Buenos Aires) 107, 73–74.Google Scholar
Binsztein, N., Costagliola, M.C., Pichel, M., Jurquiza, V., Ramírez, F.C., Akselman, R., Vacchino, M., Huq, A. and Colwell, R. (2004) Viable but nonculturable Vibrio cholerae O1 in the aquatic environment of Argentina. Applied and Environmental Microbiology 70, 7481–7486.CrossRefGoogle ScholarPubMed
Chowdhury, M.A.R., Xu, B., Montilla, R., Hasan, J.A.K., Huq, A. and Colwell, R.R. (1995) A simplified immunofluorescence technique for detection of viable cells of Vibrio cholerae O1 and O139. Journal of Microbiological Methods 24, 165–170.CrossRefGoogle Scholar
Chun, J., Huq, A. and Colwell, R. (1999) Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholerae and Vibrio mimicus. Applied and Environmental Microbiology 65, 2202–2208.CrossRefGoogle ScholarPubMed
Colwell, R.R. (1996) Global climate and infectious disease: the cholera paradigm. Science 274, 2025–2031.CrossRefGoogle ScholarPubMed
Colwell, R.R. and Huq, A. (1994) Vibrios in the environment: viable but nonculturable Vibrio cholerae. In Wachsmuth, I.K., Blake, P.A. and Olsvik, Ø. (eds) Vibrio cholerae and cholera: molecular to global perspectives. Washington, DC: American Society for Microbiology, pp. 117–133.Google Scholar
Costagliola, M., López, A., Malaspina, A., Guerrero, R., Medina, D., Odizzio, M., Abelenda, A. and Friss de Kereki, C. (2000) Estudio de la presencia de Vibrio cholerae en la Zona Común de Pesca Argentino–Uruguaya. Período 1992–1996. Frente Marítimo 18, 53–58.Google Scholar
Cottingham, K.L., Chiavelli, D.A. and Taylor, R.K. (2003) Environmental microbe and human pathogen: the ecology and microbiology of Vibrio cholerae. Frontiers in Ecology and the Environment 1, 80–86.CrossRefGoogle Scholar
Dela-Cruz, J., Ajani, P., Lee, R., Pritchard, T. and Suthers, I. (2002) Temporal abundance of the red tide dinoflagellate Noctiluca scintillans along the southeast coast of Australia. Marine Ecology Progress Series 236, 75–88.CrossRefGoogle Scholar
González Fraga, S., Pichel, M., Costagliola, M.C., Cecilia, M., Jurquiza, V., Peressutti, S., Caffer, M.I., Aulet, O., Hozbor, C., Tracanna, B.C., Villagra de Gamundi, A., Hernández, D., Ramírez, F.C., Akselman, R. and Binsztein, N. (2007) Environment and virulence factors of Vibrio cholerae strains isolated in Argentina. Journal of Microbiology 103, 2448–2456.Google Scholar
Islam, M.S., Drasar, B.S. and Sack, R.B. (1996) Ecology of Vibrio cholerae: role of aquatic fauna and flora. In Drasar, B.S. and Forrest, B.D. (eds) Cholera and the ecology of Vibrio cholerae. London: Chapman and Hall, pp. 187–227.CrossRefGoogle Scholar
Islam, M.S., Rahim, Z., Alam, M.J., Begum, S., Moniruzzaman, S.M., Umeda, A., Amako, K., Albert, M.J., Sack, R.B., Huq, A. and Colwell, R.R. (1999) Association of Vibrio cholerae O1 with the cyanobacterium Anabaena sp., elucidated by polymerase chain reaction and transmission electron microscopy. Transactions of the Royal Society of Tropical Medicine and Hygiene 93, 36–40.CrossRefGoogle ScholarPubMed
Mouriño-Pérez, R.R., Worden, A.Z. and Azam, F. (2003) Growth of Vibrio cholerae O1 in red tide waters off California. Applied and Environmental Microbiology 69, 6923–6931.CrossRefGoogle ScholarPubMed
Mueller, R.S., McDougald, D., Cusumano, D., Sodhi, N., Kjelleberg, S., Azam, F. and Bartlett, D.H. (2007) Vibrio cholerae strains possess multiple strategies for abiotic and biotic surface colonization. Journal of Bacteriology 189, 5348–5360.CrossRefGoogle ScholarPubMed
Murray, S. and Suthers, I.M. (1999) Population ecology of Noctiluca scintillans Macartney, a red-tide forming dinoflagellate. Marine and Freshwater Research 50, 243–252.CrossRefGoogle Scholar
Pichel, M., Rivas, M., Martin, F., Ibarra, C. and Binsztein, N. (2003) Genetic diversity and emergence of a new variant of Vibrio cholerae O1 isolated in Argentina. Journal of Clinical Microbiology 41, 124–134.CrossRefGoogle ScholarPubMed
Rivera, I., Chun, J., Huq, A., Sack, R. and Colwell, R.R. (2001) Genotypes associated with virulence in environmental isolates of Vibrio cholerae. Applied and Environmental Microbiology 67, 2421–2429.CrossRefGoogle ScholarPubMed
Rivera, I., Lipp, E., Gil, A., Choopun, N., Huq, A. and Colwell, R.R. (2003) Method for extraction and application of Multiplex PCR to detect toxigenic V. cholerae O1 and O139 from aquatic ecosystems. Environmental Microbiology 5, 599–606.CrossRefGoogle ScholarPubMed
Tamplin, M.L., Gauzens, A.L., Huq, A., Sack, D.A. and Colwell, R.R. (1990) Attachment of Vibrio cholerae serogroup O1 to zooplankton and phytoplankton of Bangladesh waters. Applied and Environmental Microbiology 56, 1977–1980.CrossRefGoogle ScholarPubMed
Uhlig, G. and Sahling, G. (1990) Long-term studies on Noctiluca scintillans in the German Bight. Population dynamics and red tide phenomena 1968–1988. Netherlands Journal of Sea Research 25, 101–112.CrossRefGoogle Scholar