Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-26T02:16:01.188Z Has data issue: false hasContentIssue false
  • English
  • Français

A new record of the Kumamoto oyster Crassostrea sikamea in the Seto Inland Sea, Japan

Published online by Cambridge University Press:  13 February 2013

Masami Hamaguchi ,
Hiromori Shimabukuro ,
Masako Kawane  and
Tomoki Hamaguchi
Masami Hamaguchi*
Affiliation:
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan
Hiromori Shimabukuro
Affiliation:
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan
Masako Kawane
Affiliation:
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan
Tomoki Hamaguchi
Affiliation:
Itsukaichi Minami Junior High school, Kairouen 4-2-21, Hiroshima 731-5135, Japan
*
Correspondence should be addressed to: M. Hamaguchi, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan email:[email protected]
Get access

Abstract

We collected 138 Kumamoto like oyster specimens in and around the Nakatsu tidal flat to monitor species diversity. The mitochondrial large subunit of ribosomal DNA (LSrDNA) and the nuclear ribosomal internally transcribed spacer-1 (ITS-1) region were amplified by polymerase chain reaction and restriction fragment length polymorphism analysis, leading to the discovery of the Kumamoto oyster Crassostrea sikamea in the Nakatsu tidal flat. A more accurate species identification using the nucleotide sequences for cytochrome c oxidase subunit I and ITS-1 confirmed the presence of the oysters. These results provide the first evidence of C. sikamea in the Seto Inland Sea of Japan.

Keywords

Crassostrea sikameapolymerase chain reaction–restriction fragment length polymorphismnucleotide sequenceSeto Inland SeaJapan
Type
Research Article
Information
Marine Biodiversity Records , Volume 6 , 2013 , e16
Copyright
Copyright © Marine Biological Association of the United Kingdom 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ahmed, M. (1975) Speciation in living oysters. Advances in Marine Biology 13, 375–397.Google Scholar
Amemiya, I. (1928) Ecological studies of Japanese oysters, with specific reference to the salinity of their habitats. Journal of the College of Agriculture, Imperial University of Tokyo 9, 333–382.Google Scholar
Aranishi, F. and Iidzuka, Y. (2007) Multiplex PCR diagnosis for Crassostrea oyster discrimination of C. sikamea and C. gigas . Journal of Fisheries and Aquatic Science 2, 173–177.Google Scholar
Banks, M.A., Waters, C. and Hedgecock, D. (1993) Discrimination between closely related Pacific oysters (Crassostrea) via mitochondrial DNA sequences coding for large subunit rRNA. Molecular Marine Biology and Biotechnology 2, 129–136.Google Scholar
Banks, M.A., McGoldrick, D.J., Borgeson, W. and Hedgecock, D. (1994) Gametic incompatibility and genetic divergence of pacific and Kumamoto oysters, Crassostrea gigas and C. sikamea . Marine Biology 121, 127–135.Google Scholar
Camara, M.D., Davis, J.P., Sekino, M., Hedgecock, D., Li, G., Langdon, C.J. and Evans, S. (2008) The Kumamoto oyster Crassostrea sikamea is neither rare nor threatened by hybridization in the northern Ariake Sea, Japan. Journal of Shellfish Research 27, 313–322.Google Scholar
Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.Google ScholarPubMed
Hedgecock, D., Banks, M.A. and McGoldrick, D.J. (1993) The status of the Kumamoto oyster Crassostrea sikamea (Amemiya 1928) in US commercial brood stocks. Journal of Shellfish Research 12, 215–221.Google Scholar
Hedgecock, D., Li, G., Banks, M.A. and Kain, Z. (1999) Occurrence of the Kumamoto oyster Crassostrea sikamea in the Ariake Sea, Japan. Marine Biology 133, 65–68.Google Scholar
Huelsenbeck, J.P. and Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17, 754–755 Google Scholar
Imai, T. and Sakai, S. (1961) Study of breeding the Japanese oyster, Crassostrea gigas . Tohoku Journal of Agriculture Research 12, 125–171.Google Scholar
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. and Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947–2948.CrossRefGoogle ScholarPubMed
Liu, J., Li, Q., Kong, L., Yu, H. and Zheng, X. (2011) Identifying the true oysters (Bivalvia: Osteidae) with mitochondrial phylogeny and distance-based DNA barcoding. Molecular Ecology Resources 11, 820–830.Google Scholar
Nakano, H. (2007) Kumamoto oyster no ohanashi (What's a kumamoto oyster?). Kumamoto Suiken Center News 15, 9. [In Japanese.]Google Scholar
Numachi, K. (1971) Biological research on the oyster. In Imai, T. (ed.) Aquaculture in shallow seas: progress in shallow sea culture. Tokyo: Koseisha Koseikaku, pp. 82–105. [In Japanese.]Google Scholar
Okoshi, K. (2004) Alien species introduced with imported clams: the clam-eating moon snail Euspira fortunei and other unintentionally introduced species. Japanese Journal of Benthology 59, 74–82. [In Japanese with English abstract.]Google Scholar
Reece, K.S., Cordes, J.F., Stubbs, J.B., Hudson, K.I. and Francis, F.A. (2008) Molecular phylogenies help resolve taxonomic confusion with Asian Crassostrea oyster species. Marine Biology 153, 709–721.Google Scholar
Ren, J.F., Liu, X., Zhang, G.F., Guo, X.M. and Liu, B. (2010) Unusual conservation of mitochondrial gene order in Crassostrea oysters: evidence for recent speciation in Asia. BMC Evolutionary Biology 10, 394.Google Scholar
Ronquist, F. and Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 20, 407–415.Google Scholar
Robinson, A. (1992) Gonadal cycle of Crassostrea gigas sikamea (Thunberg) in Yaquina Bay, Oregon and optimum conditions for broodstock oysters and larval culture. Aquaculture 106, 89–97.Google Scholar
Sekino, M. (2009) In search of the Kumamoto oyster Crassostrea sikamea (Amemiya, 1928) based on molecular markers: is the natural resource at stake? Fisheries Science 75, 819–831.Google Scholar
Simpson, J.H. (1997) Physical processes in the ROFI regime. Journal of Marine Systems 12, 3–15.Google Scholar
Sou, T. (2008) Shikame-gaki youshoku eno trikumi—Kumamoto oyster no fukkatsu (Aquaculture trials of Kumamoto oyster in Kumamoto, Japan—the wise uses of native Kumamoto oyster.). Kumamoto Suiken Center News 16, 3. [In Japanese.]Google Scholar
Tanabe, A.S. (2011) Kakusan4 and Aminosan: two programs for comparing non-partitioned, proportional, and separate models for combined molecular phylogenetic analyses of multilocus sequence data. Molecular Ecology Resources 11, 914–921 Google Scholar
Torigoe, K. (1981) Oysters in Japan. Journal of Science—Hiroshima University Series B Division 1 (Zoology) 29, 291–481.Google Scholar
Usuki, H. (2002) Evaluation of characteristics and preservation of Pacific oyster, Crassostrea gigas, in view of the genetic resources. Bulletin of Fisheries Research Agency 4, 40–104. [In Japanese with English abstract.]Google Scholar
Wang, H. and Guo, X. (2008) Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR. Journal of Shellfish Research 27, 481–487.Google Scholar
Woelke, C.E. (1955) Introduction of the Kumamoto oyster Ostrea (Crassostrea) gigas to the Pacific coast. Washington Department of Fisheries Research Papers 1, 41–50.Google Scholar
Wu, X.Y., Xu, X.D., Yu, Z.N., Wei, Z.P. and Xia, J.J. (2010) Comparison of seven Crassostrea mitogenomes and phylogenetic analyses. Molecular Phylogenetics and Evolution 57, 228–254.Google Scholar