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High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances

Published online by Cambridge University Press:  28 March 2007

Ulf Arup ,
Stefan Ekman ,
Louise Lindblom  and
Jan-Eric Mattsson
Ulf Arup
Affiliation:
Department of Systematic Botany, University of Lund, Ö. Vallgatan 18, S-223 61 Lund, Sweden.
Stefan Ekman
Affiliation:
Department of Systematic Botany, University of Lund, Ö. Vallgatan 18, S-223 61 Lund, Sweden.
Louise Lindblom
Affiliation:
Department of Systematic Botany, University of Lund, Ö. Vallgatan 18, S-223 61 Lund, Sweden.
Jan-Eric Mattsson
Affiliation:
Department of Systematic Botany, University of Lund, Ö. Vallgatan 18, S-223 61 Lund, Sweden.
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Abstract

High performance thin layer chromatography (HPTLC) is a method that can be used for screening lichen substances. It is as simple to use as standard TLC, but has many advantages: It is more sensitive, it is possible to run more samples in a shorter period of time, and the amount of solvent used is much smaller. The material needed and the methods used are described in detail. Horizontal chromatogram development was used. Since two of the solvents used in system B have been substituted, and since the properties of the HPTLC plates are slightly different, our results are not entirely in accordance with the standardized TLC method. A revised table for the identification of 69 lichen substances (obtained from 62 taxa) is accordingly presented.

Type
Research Article
Information
The Lichenologist , Volume 25 , Issue 1 , January 1993 , pp. 61 - 71
Copyright
Copyright © British Lichen Society 1993

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