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Gas chromatographic analysis to compare the fatty acid composition of fifteen lichen species, with a focus on Stereocaulon

Published online by Cambridge University Press:  28 July 2016

Thi Huyen VU ,
Daniel CATHELINE ,
David DELMAIL ,
Joël BOUSTIE ,
Philippe LEGRAND  and
Françoise LOHÉZIC-LE DÉVÉHAT
Thi Huyen VU
Affiliation:
UMR CNRS 6226 ISCR, Equipe PNSCM, Université de Rennes 1, 35043 Rennes Cedex, France
Daniel CATHELINE
Affiliation:
Laboratoire de Biochimie, Institut National de la Recherche Agronomique, USC 1378, Agrocampus-Ouest, 35042 Rennes Cedex, France
David DELMAIL
Affiliation:
UMR CNRS 6226 ISCR, Equipe PNSCM, Université de Rennes 1, 35043 Rennes Cedex, France
Joël BOUSTIE
Affiliation:
UMR CNRS 6226 ISCR, Equipe PNSCM, Université de Rennes 1, 35043 Rennes Cedex, France
Philippe LEGRAND
Affiliation:
Laboratoire de Biochimie, Institut National de la Recherche Agronomique, USC 1378, Agrocampus-Ouest, 35042 Rennes Cedex, France
Françoise LOHÉZIC-LE DÉVÉHAT*
Affiliation:
UMR CNRS 6226 ISCR, Equipe PNSCM, Université de Rennes 1, 35043 Rennes Cedex, France
*
Email: [email protected]
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Abstract

The composition of fatty acids (FAs) is known to discriminate families within genera of bacteria, fungi, octocorals and algae. Here, we applied the more sensitive gas chromatographic (GC) analysis to investigate the taxonomic value of using FA composition to discriminate 15 species of lichen, including three chlorolichens (two Cladonia species and Stereocaulon scutelligerum), nine tripartite lichens (Stereocaulon species) and three cyanolichens (Lichina pygmaea, Collema cristatum, Peltigera membranacea). One macroscopic cyanobacterium (Nostoc sp.), corresponding to the photobiont partner of Peltigera and Collema cyanolichens, was included for comparison. Five lipid extraction methods were evaluated, using Stereocaulon scutelligerum. Shaking at 80 °C with chloroform/methanol (2:1, v/v) was the most efficient and reproducible method. The total FA composition of the 16 species was compared, using GC coupled with flame ionization detection or mass spectrometry. A statistical hierarchical cluster analysis was used to determine the similarity between the FA profiles, based on total, saturated, unsaturated and branched-chain FAs (BCFAs). The BCFA profile allowed a distinction between the Stereocaulon species and the cyanolichens, in contrast to all the other types of FAs. A detailed characterization of eight FA subclasses, provided by the GC analysis, suggested a preferential pathway for the biogenesis of unsaturated FAs through desaturases, which was especially favoured in the cyanolichens.

Keywords

biosynthetic pathwaysbranched-chain fatty acidschemotaxonomycyanolichensNostoc
Type
Articles
Information
The Lichenologist , Volume 48 , Issue 4 , July 2016 , pp. 323 - 337
Copyright
© British Lichen Society, 2016 

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