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Development of microsatellite markers in Protoparmeliopsis muralis (lichenized Ascomycete) – a common lichen species

Published online by Cambridge University Press:  31 October 2013

Beata GUZOW-KRZEMIŃSKA  and
Elfie STOCKER-WÖRGÖTTER
Beata GUZOW-KRZEMIŃSKA
Affiliation:
Department of Molecular Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland; and Department of Organismic Biology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria. Email: [email protected]
Elfie STOCKER-WÖRGÖTTER
Affiliation:
Department of Organismic Biology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria
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Abstract

Lichen-forming fungi are symbiotic organisms forming a thallus with autotrophic green algae and/or cyanobacteria. Protoparmeliopsis muralis (Schreb.) Choisy is a green-algal lichen-forming fungus associating with Trebouxia photobionts. It is known as one of the most successful urban lichens in the world. In this paper, the development of microsatellite markers specific for the mycobiont of Protoparmeliopsis muralis is reported. In order to avoid algal contaminations, the pure mycobiont culture was obtained and subsequently used for DNA isolation. For DNA enrichment, the Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) method was applied. Of the 380 clones sequenced, 62 contained repeats. In total, 38 primer pairs were designed and tested, and finally 7 primer pairs were polymorphic based on 21 specimens of P. muralis.

Keywords

FIASCOlichenmycobiontpopulation
Type
Articles
Information
The Lichenologist , Volume 45 , Issue 6 , November 2013 , pp. 791 - 798
Copyright
Copyright © British Lichen Society 2013 

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