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A new method to isolate lichen algae by using percoll® gradient centrifugation

Published online by Cambridge University Press:  28 March 2007

Angeles Calatayud ,
Alfredo Guera ,
Simón Fos  and
Eva Barreno
Angeles Calatayud
Affiliation:
A. Calatayud, S. Fos & E Barreno: Universitat de Valencia, Facultad de Ciencias Biológicas, Departamentode Biologia Vegetal, C/ Dr. Mliner, 50, E-46100 Burjassot (Valencia) Spain.
Alfredo Guera
Affiliation:
A. Guera: Universidad de Alcalá de Henares, Facultad de Ciencias Biologicas, Departamento de Biologia Vegetal, E-28871 Alcalá de Henares (Madrid) Spain.
Simón Fos
Affiliation:
A. Guera: Universidad de Alcalá de Henares, Facultad de Ciencias Biologicas, Departamento de Biologia Vegetal, E-28871 Alcalá de Henares (Madrid) Spain.
Eva Barreno
Affiliation:
A. Calatayud, S. Fos & E Barreno: Universitat de Valencia, Facultad de Ciencias Biológicas, Departamentode Biologia Vegetal, C/ Dr. Mliner, 50, E-46100 Burjassot (Valencia) Spain.
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Abstract

A rapid method to isolate intact functional algae from the lichens Evemia prunastri and Ramalina farinacea has been developed. This method is based on the use of Percoll® gradients after mechanical disruption of lichen thalli. Results obtained show that the algal preparations were virtually free of contamination by fungal hyphae. The purified algal cells were photosynthetically active and without symptoms of photoinhibition, which indicates their functional integrity. This method may be used for the isolation of intact algae from a broad range of lichen species.

Type
Research Article
Information
The Lichenologist , Volume 33 , Issue 4 , July 2001 , pp. 361 - 366
Copyright
Copyright © British Lichen Society 2001

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