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Molecular, morphological and chemical variation of the Usnea pectinata aggregate from Tanzania, São Tomé and Príncipe

Published online by Cambridge University Press:  29 November 2022

Stella G. Temu Open the ORCID record for Stella G. Temu [Opens in a new window] ,
Philippe Clerc Open the ORCID record for Philippe Clerc [Opens in a new window] ,
Miko R. A. Nadel Open the ORCID record for Miko R. A. Nadel [Opens in a new window] ,
Leif Tibell Open the ORCID record for Leif Tibell [Opens in a new window] ,
Donatha D. Tibuhwa  and
Sanja Tibell Open the ORCID record for Sanja Tibell [Opens in a new window]
Stella G. Temu
Affiliation:
Department of Organismal Biology, Uppsala University (UU), Sweden Department of Molecular Biology and Biotechnology, University of Dar es Salaam (UDSM), Tanzania
Philippe Clerc
Affiliation:
Conservatoire et Jardin botaniques de la Ville de Genève (CJBG), CH-1292 Chambésy/GE, Switzerland
Miko R. A. Nadel
Affiliation:
Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, California 94132, USA
Leif Tibell
Affiliation:
Department of Organismal Biology, Uppsala University (UU), Sweden
Donatha D. Tibuhwa
Affiliation:
Department of Molecular Biology and Biotechnology, University of Dar es Salaam (UDSM), Tanzania
Sanja Tibell*
Affiliation:
Department of Organismal Biology, Uppsala University (UU), Sweden
*
Author for correspondence: Sanja Tibell. E-mail: [email protected]
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Abstract

This study investigated the molecular, chemical and morphological variation in the Usnea pectinata aggregate using 42 specimens, 22 from Tanzania and 20 from São Tomé and Príncipe. A total of 31 sequences (13 ITS, 13 nuLSU and 5 RPB1) were generated. The results are presented in two phylogenies: first a three-markers ‘backbone’ phylogeny for the U. pectinata aggregate, where six distinct, strongly supported subclades indicate considerable genetic variation in the dataset; and second, an ITS phylogeny with 47 terminals along with a mapping of morphological and chemistry data. Several well-supported monophyletic clades were recovered in both phylogenies and these may well represent separate species in the complex referred to here as the U. pectinata aggregate. Three morphotypes characterized by axis pigmentation and four by branch shape were noted. Six chemotypes were observed.

Keywords

chemistrylichenmolecular phylogenyParmeliaceaetaxonomy
Type
Standard Paper
Information
The Lichenologist , Volume 54 , Special Issue 5: African Lichenology , September 2022 , pp. 291 - 298
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the British Lichen Society

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