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Ammonium, nitrate and glycine uptake of six Ecuadorian tropical montane forest tree species: an in situ pot experiment with saplings

Published online by Cambridge University Press:  26 November 2014

Bärbel Wittich ,
Jürgen Homeier  and
Christoph Leuschner
Bärbel Wittich
Affiliation:
Plant Ecology, Albrecht von Haller Institute for Plant Sciences, University of Göttingen, Untere Karspüle 2, 37073 Göttingen, Germany
Jürgen Homeier
Affiliation:
Plant Ecology, Albrecht von Haller Institute for Plant Sciences, University of Göttingen, Untere Karspüle 2, 37073 Göttingen, Germany
Christoph Leuschner*
Affiliation:
Plant Ecology, Albrecht von Haller Institute for Plant Sciences, University of Göttingen, Untere Karspüle 2, 37073 Göttingen, Germany
*
1Corresponding author. Email: [email protected]
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Abstract:

Not much is known about the nitrogen (N) uptake capacity and N-form preference of tropical trees. In a replicated labelling experiment with 15N-ammonium, 15N-nitrate and dual-labelled glycine applied to saplings of six tree species from southern Ecuadorian montane forests, we tested the hypotheses that (1) the saplings of tropical trees are capable of using organic N even though they are forming arbuscular mycorrhizas, and (2) with increasing altitude, tree saplings increasingly prefer ammonium and glycine over nitrate due to reduced nitrification and growing humus accumulation. Three- to 5-y-old saplings of two species each from 1000, 2000 and 3000 m asl were grown in pots inside the forest at their origin and labelled with non-fertilizing amounts of the three N forms; 15N enrichment was detected 5 days after labelling in fine roots, coarse roots, shoots and leaves. The six species differed with respect to their N-form preference, but neither the abundance of ammonium and nitrate in the soil nor altitude (1000–3000 m asl) seemed to influence the preference. Two species (those with highest growth rate) preferred NH4+ over NO3−, while the other four species took up NO3− and NH4+ at similar rates when both N forms were equally available. After 13C-glycine addition, 13C was significantly accumulated in the biomass of three species (all species with exclusively AM symbionts) but a convincing proof of the uptake of intact glycine molecules by these tropical montane forest trees was not obtained.

Keywords

altitudinal gradientdual-labelled glycineGraffenrieda harlingiiHedyosmum purpurascensHedyosmum spruceiHedyosmum translucidumMyrcia sp. nov.nitrogen uptakePouteria torta15N tracer study
Type
Research Article
Information
Journal of Tropical Ecology , Volume 31 , Issue 2 , March 2015 , pp. 139 - 152
Copyright
Copyright © Cambridge University Press 2014 

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References

LITERATURE CITED

ANDERSEN, K. M. & TURNER, B. L. 2013. Preferences or plasticity in nitrogen acquisition by understorey palms in a tropical montane forest. Journal of Ecology 101:819–825.Google Scholar
ARNDT, S. K., WANEK, W., HOCH, G., RICHTER, A. & POPP, M. 2002. Flexibility of nitrogen metabolism in the tropical C3–crassulacean acid metabolism tree species Clusia minor. Functional Plant Biology 29:741–747.CrossRefGoogle ScholarPubMed
AVERILL, C. & FINZI, A. 2011. Increasing plant use of organic nitrogen with elevation is reflected in nitrogen uptake rates and ecosystem δ15N. Ecology 92:883–891.Google Scholar
BENDIX, J., HOMEIER, J., CUEVA ORTIZ, E., EMCK, P., BRECKLE, S.-W., RICHTER, M. & BECK, E. 2006. Seasonality of weather and tree phenology in a tropical evergreen mountain rain forest. International Journal of Biometeorology 50:370–384.Google Scholar
BREARLEY, F. Q. 2012. Nitrogen stable isotopes indicate differences in nitrogen cycling between two contrasting Jamaican montane forests. Plant and Soil 367:465–476.Google Scholar
DI, H. J. & CAMERON, K. C. 2004. Effects of temperature and application rate of a nitrification inhibitor, dicyandiamide (DCD), on nitrification rate and microbial biomass in a grazed pasture soil. Australian Journal of Soil Research 42:927–932.Google Scholar
FINZI, A. C. & BERTHRONG, S. T. 2005. The uptake of amino acids by microbes and trees in three cold-temperate forests. Ecology 86:3345–3353.CrossRefGoogle Scholar
GOLLER, R., WILCKE, W., FLEISCHBEIN, K., VALAREZO, C. & ZECH, W. 2006. Dissolved nitrogen, phosphorus, and sulfur forms in the ecosystem fluxes of a montane forest in Ecuador. Biogeochemistry 77:57–89.CrossRefGoogle Scholar
GRAEFE, S., LEUSCHNER, C., CONERS, H. & HERTEL, D. 2011. Root functioning in tropical high-elevation forests: environmental vs. biological control of root water absorption. Environmental and Experimental Botany 71:329–336.Google Scholar
GUGGENBERGER, G., ZECH, W. & SCHULTEN, H.-R. 1994. Formation and mobilization pathways of dissolved organic matter: evidence from chemical structural studies of organic matter fractions in acid forest floor solutions. Organic Geochemistry 21:51–66.CrossRefGoogle Scholar
HOMEIER, J., WERNER, F. A., GRADSTEIN, S. R., BRECKLE, S.-W. & RICHTER, M. 2008. Potential vegetation and floristic composition of Andean forests in South Ecuador, with a focus on the RBSF. Pp. 87–100 in Beck, E., Bendix, J., Kottke, I., Makeschin, F. & Mosandl, R. (eds.). Gradients in a tropical mountain ecosystem of Ecuador. Ecological Studies Vol. 198, Springer Verlag, Berlin.Google Scholar
JACOB, A. & LEUSCHNER, C. 2014. Complementarity in the use of nitrogen forms in temperate broad-leaved mixed forest. Plant Ecology and Diversity, in press.Google Scholar
JONES, D. L., KIELLAND, K., SINCLAIR, F. L., DAHLGREN, R. A., NEWSHAM, K. K., FARRAR, J. F. & MURPHY, D. V. 2009. Soil organic nitrogen mineralization across a global latitudinal gradient. Global Biogeochemical Cycles 23: GB1016.Google Scholar
KIELLAND, K. 1994. Amino acid absorption by arctic plants: implications for plant nutrition and nitrogen cycling. Ecology 75:2373.Google Scholar
KIELLAND, K. 1997. Role of free amino acids in the nitrogen economy of arctic cryptogams. Ecoscience 4:75–79.Google Scholar
KOTTKE, I., BECK, A., OBERWINKLER, F., HOMEIER, J. & NEILL, D. 2004. Arbuscular endomycorrhizas are dominant in the organic soil of a neotropical montane cloud forest. Journal of Tropical Ecology 20:125–129.CrossRefGoogle Scholar
MARRS, R. H., PROCTOR, J., HEANEY, A. & MOUNTFORD, M. D. 1988. Changes in soil nitrogen-mineralization and nitrification along an altitudinal transect in tropical rain forest in Costa Rica. Journal of Ecology 76:466–482.Google Scholar
MICHALZIK, B., KALBITZ, K., PARK, J.-H., SOLINGER, S. & MATZNER, E. 2001. Fluxes and concentrations of dissolved organic carbon and nitrogen – a synthesis for temperate forests. Biogeochemistry 52:173–205.Google Scholar
MOSER, G., HERTEL, D. & LEUSCHNER, C. 2007. Altitudinal change in LAI and stand leaf biomass in tropical montane forests: a transect study in Ecuador and a pan-tropical meta-analysis. Ecosystems 10:924–935.Google Scholar
MOSER, G., LEUSCHNER, C., HERTEL, D., GRAEFE, S., SOETHE, N. & IOST, S. 2011. Elevation effects on the carbon budget of tropical mountain forests (S Ecuador): the role of the belowground compartment. Global Change Biology 17:2211–2226.Google Scholar
NÄSHOLM, T. & PERSSON, J. 2001. Plant acquisition of organic nitrogen in boreal forests. Physiologia Plantarum 111:419–426.CrossRefGoogle ScholarPubMed
NÄSHOLM, T., EKBLAD, A., NORDIN, A., GIESLER, R., HÖGBERG, M. & HÖGBERG, P. 1998. Boreal forest plants take up organic nitrogen. Nature 392:914–916.CrossRefGoogle Scholar
PAOLI, G. D., CURRAN, L. M. & ZAK, D. R. 2005. Phosphorus efficiency of Bornean rain forest productivity: evidence against the unimodal efficiency hypothesis. Ecology 86:1548–1561.Google Scholar
RASMUSSEN, J. & KUZYAKOV, Y. 2009. Carbon isotopes as proof for plant uptake of organic nitrogen: relevance of inorganic carbon uptake. Soil Biology and Biochemistry 41:1586–1587.Google Scholar
TANNER, E. V. J. 1981. The decomposition of leaf litter in Jamaican montane rain forests. Journal of Ecology 69:263–275.Google Scholar
TANNER, E. V. J., VITOUSEK, P. M. & CUEVAS, E. 1998. Experimental investigation of nutrient limitation of forest growth on wet tropical mountains. Ecology 79:10–22.Google Scholar
USELMAN, S. M., QUALLS, R. G. & LILIENFEIN, J. 2012. Quality of soluble organic C, N, and P produced by different types and species of litter: root litter versus leaf litter. Soil Biology and Biochemistry 54:57–67.Google Scholar
VERMA, A., TYAGI, L. & SINGH, S. N. 2007. Attenuation of N2O emission rates from agricultural soil at different dicyandiamide concentrations. Environmental Monitoring and Assessment 137:287–293.Google Scholar
VITOUSEK, P. M. & SANFORD, R. L. 1986. Nutrient cycling in moist tropical forest. Annual Review of Ecology and Systematics 17:137–167.CrossRefGoogle Scholar
WANEK, W. & PÖRTL, K. 2008. Short-term 15N uptake kinetics and nitrogen nutrition of bryophytes in a lowland rainforest, Costa Rica. Functional Plant Biology 35:51–62.CrossRefGoogle Scholar
WANEK, W., ARNDT, S. K., HUBER, W. & POPP, M. 2002. Nitrogen nutrition during ontogeny of hemiepiphytic Clusia species. Functional Plant Biology 29:733–740.Google Scholar
WITTICH, B., HORNA, V., HOMEIER, J. & LEUSCHNER, C. 2012. Altitudinal change in the photosynthetic capacity of tropical trees – a case study from Ecuador and a pantropical literature analysis. Ecosystems 15:958–973.Google Scholar
WOLF, K., VELDKAMP, E., HOMEIER, J. & MARTINSON, G. O. 2011. Nitrogen availability links forest productivity, soil nitrous oxide and nitric oxide fluxes of a tropical montane forest in southern Ecuador. Global Biogeochemical Cycles 25, GB4009. doi: 10.1029/2010GB003876, 2011.Google Scholar
ZACHERL, B. & AMBERGER, A. 1990. Effect of the nitrification inhibitors dicyandiamide, nitrapyrin and thiourea on Nitrosomonas europaea. Fertilizer Research 22:37–44.CrossRefGoogle Scholar