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Stress responses of the green microalga, Dunaliella salina to PEG-induced drought

Published online by Cambridge University Press:  29 October 2020

Fatemeh Tafvizi ,
Seyed Ali Hosseini Tafreshi Open the ORCID record for Seyed Ali Hosseini Tafreshi [Opens in a new window] ,
Zeinab Toluei  and
Mohammad Amin Toghyani
Fatemeh Tafvizi
Affiliation:
Biotechnology Division, Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
Seyed Ali Hosseini Tafreshi*
Affiliation:
Biotechnology Division, Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
Zeinab Toluei
Affiliation:
Biotechnology Division, Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
Mohammad Amin Toghyani
Affiliation:
Biotechnology Division, Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
*
Author for correspondence: Seyed Ali Hosseini Tafreshi, E-mail: [email protected]
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Abstract

Drought stress was evaluated with polyethylene glycol 6000 (PEG 6000) treatment in Dunaliella salina, a microalga known for its great ability to withstand salinities of more than 30%. The aim was to explore the acclimation mechanisms used by the microalga to regulate its growth and physiology during coping with drought stress. The microalga was subjected to culture mediums containing 2 and 5% PEG for 25 days and was compared with a control culture medium. Significant decrease in growth parameters such as specific growth rate, biomass and number of divisions per day was demonstrated in PEG-treated algae. During PEG treatment, chlorophylls slightly increased, while β-carotene and total protein were not affected. Osmolytes, as well as carbohydrates, were found to be significantly higher in PEG-treated algae than in control. Increased catalase and ascorbate peroxidase activities were proportionally related to PEG concentrations in the cultures. The PEG-treated cells accumulated a considerable amount of hydrogen peroxide and malondialdehyde, especially at higher PEG concentrations. Electrolyte leakage increased, regardless of the PEG concentrations applied, while DNA fragmentation was not observed after 25 days of treating with PEG. It was concluded that Dunaliella cells could respond to the drought stress, probably by using a higher accumulation of a range of osmolytes and also more stimulation of the antioxidant enzymatic system.

Keywords

Acclimation to droughtalgaeeffects-physiologygrowthpolyethylene glycol
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 100 , Issue 7 , November 2020 , pp. 1043 - 1052
Copyright
Copyright © Marine Biological Association of the United Kingdom 2020

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