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Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

Published online by Cambridge University Press:  19 May 2016

Elangovan Dilipan ,
Jutta Papenbrock  and
Thirunavakkarasu Thangaradjou
Elangovan Dilipan
Affiliation:
Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai – 608 502, Cuddalore Dt., Tamilnadu, India
Jutta Papenbrock
Affiliation:
Institute of Botany, Leibniz University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany
Thirunavakkarasu Thangaradjou*
Affiliation:
Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai – 608 502, Cuddalore Dt., Tamilnadu, India
*
Correspondence should be addressed to:T. Thangaradjou, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai – 608 502, Cuddalore Dt., Tamilnadu, India email: [email protected]
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Abstract

In India 14 seagrass species can be found with monospecific genera (Enhalus, Thalassia and Syringodium), Cymodocea with two species and Halophila and Halodule represented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of the Halophila and Halodule complex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.

Keywords

Marine angiospermgenetic variabilityRAPDIndia
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 6 , September 2017 , pp. 1307 - 1315
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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Footnotes

*

Present address: Science and Engineering Research Board, New Delhi 110070.

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