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A non-invasive technique for obtaining DNA from marine intertidal snails

Published online by Cambridge University Press:  12 August 2004

Kei Kawai ,
Motohiro Shimizu ,
Roger N. Hughes  and
Osamu Takenaka
Kei Kawai
Affiliation:
Research Center for the Pacific Islands, Kagoshima University, Korimoto 1-21-24, Kagoshima, 890-8580, Japan School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK
Motohiro Shimizu
Affiliation:
Comparative Physiology Laboratory, Faculty of Fisheries, Hokkaido University, Minato 3-1-1, Hakodate, 041-8611, Japan
Roger N. Hughes
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK
Osamu Takenaka
Affiliation:
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Kanrin Inuyama, Aichi, 484-8005, Japan
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Abstract

DNA was extracted from mucus secreted by snails that had been allowed to crawl over glass microscope slides. The mucus contained many epithelial cells and a few blood cells. Microsatellite DNA regions were amplified using template DNA from the mucus and clear bands obtained showing the same positions as when using template DNA from the foot. Pedal mucus is therefore a reliable source of DNA, which can be extracted by a simple methodology that is readily applied in the field. The technique has considerable potential for conservation- and behavioural ecology.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 84 , Issue 4 , August 2004 , pp. 773 - 774
Copyright
2004 Marine Biological Association of the United Kingdom

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