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Genetic variation and population structure of Holothuria polii from the eastern and western Mediterranean coasts in Tunisia

Published online by Cambridge University Press:  07 April 2011

Aicha Gharbi  and
Khaled Said
Aicha Gharbi*
Affiliation:
Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, UR03ES09, Institut Supérieur de Biotechnologie de Monastir, Tunisie Station de Biologie Marine du Muséum National d'Histoire Naturelle, BP225, 29900 Concarneau, France
Khaled Said
Affiliation:
Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, UR03ES09, Institut Supérieur de Biotechnologie de Monastir, Tunisie
*
Correspondence should be addressed to: A. Gharbi, Station de Biologie Marine du Muséum National d'Histoire Naturelle, BP225, 29900 Concarneau, France emails: [email protected]; [email protected]
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Abstract

Seven populations of Holothuria polii were sampled from the eastern and western Mediterranean coastal waters of Tunisia and screened electrophoretically for genetic variation at 11 allozyme loci. Six among the seven polymorphic loci were out of Hardy–Weinberg equilibrium (HWE) in at least one population. In the same way, the multilocus test showed deviation from HWE in all populations. These populations showed heterozygote deficiency. Genetic variability was relatively low. The number of alleles per locus ranged from 2.09 to 2.27 (average = 2.15), and the observed heterozygosity varied between 0.14 and 0.20 (average = 0.17). The observed overall differentiation among populations was slight but significant, with a mean FST value of 0.024 (P < 0.001). Pairwise FST values reflected the differentiation of the two populations, which were at the margins of the range sampled, from all the others. Our data suggest a population structure consistent with separation by Mediterranean Sea basins that might reflect different local biogeographical zones.

Keywords

allozymesgenetic differentiationHolothuria poliiMediterranean SeaTunisia
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 91 , Issue 8 , December 2011 , pp. 1599 - 1606
Copyright
Copyright © Marine Biological Association of the United Kingdom 2011

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