Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-28T03:04:33.088Z Has data issue: false hasContentIssue false
  • English
  • Français

Egg volume, energy content and fatty acid profile of Maja brachydactyla (Crustacea: Brachyura: Majidae) during embryogenesis

Published online by Cambridge University Press:  08 July 2008

Joana Figueiredo  and
Luís Narciso
Joana Figueiredo*
Affiliation:
Laboratório Marítimo da Guia, Faculdade de Ciências da Universidade de Lisboa, Avenida Nossa Senhora do Cabo, 939 2750–374 Cascais, Portugal
Luís Narciso
Affiliation:
Laboratório Marítimo da Guia, Faculdade de Ciências da Universidade de Lisboa, Avenida Nossa Senhora do Cabo, 939 2750–374 Cascais, Portugal
*
Correspondence should be addressed to: Joana Figueiredo, Laboratório Marítimo da Guia, Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa, Avenida Nossa Senhora do Cabo, 939, 2750–374 CascaisPortugal email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Fatty-acid and energy content of Maja brachydactyla eggs at different developmental stages (recently spawned, half-developed and ready to hatch) were analysed in order to understand what is being consumed and produced during the embryonic development. Egg volume increased during development (34%, 0.187 to 0.285 mm3, N = 270) and was negatively correlated with egg energy and fatty-acid content (r = −0.80 and r = −0.46, respectively), which decreased through embryogenesis. The most consumed fatty acids were the PUFA (21.2 μg · mg dw−1), followed by the SFA (18.8 μg · mg dw−1) and MUFA (14.9 μg · mg dw−1). Palmitic (16:0), oleic (18:1n-9) and eicosapentaenoic (EPA, 20:5n-3) acids were preferentially consumed (13.14, 9.21 and 8.67 μg · mg dw−1, respectively). The fatty acid composition found in M. brachydactyla eggs reflected the habitat and omnivorous and detritivorous scanvenger diet of the adults, although the consumption of algae was more important than previously thought, at least in the area where these adults were captured. Pre-hatching eggs have a high PUFA content (64.5 μg · mg dw−1 or 46.3% of the egg fatty-acid content). We conclude that larvae of this species might need a diet rich in PUFA, particularly EPA and DHA, for successful development. From the culture perspective, live preys commonly used in aquaculture will likely require to be enriched with DHA.

Keywords

crabdevelopmentecologyfatty acidlarval dietreproduction
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 88 , Issue 7 , November 2008 , pp. 1401 - 1405
Copyright
Copyright © Marine Biological Association of the United Kingdom 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Amsler, M.O. and George, R.Y. (1984) Seasonal variation in the biochemical composition of the embryos of Callinectes sapidus Rathbun. Journal of Crustacean Biology 4, 546–553.CrossRefGoogle Scholar
Andrés, M., Estévez, A. and Rotllant, G. (2007) Growth, survival and biochemical composition of spider crab Maja brachydactyla (Balss, 1922) (Decapoda: Majidae) larvae reared under different stocking densities, prey:larva ratios and diets. Aquaculture 273, 494–502.CrossRefGoogle Scholar
Anger, K. (1995) The conquest of freshwater and land by marine crabs: adaptations in life-history patterns and larval bioenergetics. Journal of Experimental Marine Biology and Ecology 193, 119–145.Google Scholar
Anger, K. (1998) Patterns of growth and chemical composition in decapod crustacean larvae. Invertebrate Reproduction and Development 33, 159–176.Google Scholar
Auel, H., Harjes, M., da Rocha, R., Stübing, D. and Hagen, W. (2002) Lipid biomarkers indicate different ecological niches and trophic relationships of the Artic hyperiid amphipods Themisto abyssorum and T. libellula. Polar Biology 25, 374–383.CrossRefGoogle Scholar
Bernárdez, C., Freire, J. and González-Gurriarán, E. (2000) Feeding of the spider crab Maja squinado in rocky subtidal areas of the Ría de Arousa (north-west Spain). Journal of the Marine Biological Association of the United Kingdom 80, 95–102.CrossRefGoogle Scholar
Bligh, E.G. and Dyer, W.J. (1959) A rapid method of total lipids extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.Google Scholar
Cahu, C., Fauvel, C. and Aquacop, (1986) Effect of food fatty acids composition of P. vannamei broodstock on egg quality. International Council for the Exploration of the Sea. Mariculture Committee 28, 1–8.Google Scholar
Clarke, A., Brown, J.H. and Holmes, L.J. (1990) The biochemical composition of eggs from Macrobrachium rosenbergii in relation to embryonic development. Comparative Biochemistry and Physiology—Part B 96, 505–511.Google Scholar
Dalsgaard, J., St John, M., Kattner, G., Müller-Navarra, D. and Hagen, W. (2003) Fatty acid trophic markers in the pelagic marine environment. Advances in Marine Biology 46, 225–340.CrossRefGoogle ScholarPubMed
De Vries, M.C. and Forward, R.B. Jr (1991) Mechanisms of crustacean egg hatching: evidence for enzyme release by crab embryos. Marine Biology 110, 281–291.Google Scholar
Ellis, J.R., Rogers, S.I. and Freeman, S.M. (2000) Demersal assemblages in the Irish Sea, St George's Channel and Bristol Channel. Estuarine, Coastal and Shelf Science 51, 299–315.CrossRefGoogle Scholar
González-Gurriarán, E., Fernández, L., Freire, J. and Muiño, R. (1998) Mating and role of seminal receptacles in the reproductive biology of the spider crab Maja squinado (Decapoda: Majidae). Journal of Experimental Marine Biology and Ecology 220, 269–285.CrossRefGoogle Scholar
Herring, P.J. (1974) Size, density and lipid content of some decapod eggs. Deep-Sea Research 21, 91–94.Google Scholar
Holland, D.L. (1978) Lipid reserves and energy metabolism in the larvae of benthic marine invertebrates. In Malins, D.C. and Sargent, J.R. (eds) Biochemical and biophysical perspectives in marine biology. London: Academic Press, pp. 85–123.Google Scholar
Kergarion, G. (1974) Régime alimentaire de Maia squinado. Conseil International pour l'Exploration de la Mer. Comité des crustacés, coquillages et benthos, CM1974/K:36, 6 pp.Google Scholar
Kergarion, G. (1984) L'araignée de mer, Maia squinado H., Biologie et exploitation. La Pêche Maritime 1279, 575–583.Google Scholar
Leme, M.H.A. (2006) Seasonal changes in reproductive traits of the crab Sesarma rectum (Grapsoidea: Sesarmidae) on the northern coast of São Paulo State, Brazil. Journal of Crustacean Biology 26, 141–147.Google Scholar
Metcalfe, L.D. and Schmitz, A.A. (1961) The rapid preparation of fatty acids esters for gass chromatography analysis. Analytical Chemistry 33, 363–364.CrossRefGoogle Scholar
Narciso, L. and Morais, S. (2001) Fatty acid profile of Palaemon serratum (Palaemonidae) eggs and larvae during embryonic and larval development using different live diets. Journal of Crustacean Biology 21, 566–574.CrossRefGoogle Scholar
Neumann, V. (1998) A review of the Maja squinado (Crustacea: Decapoda: Brachyura) species-complex with a key to the eastern Atlantic and Mediterranean species of the genus. Journal of Natural History 32, 1667–1684.Google Scholar
Oh, C. and Hartnoll, R.G. (1999) Brood loss during incubation in Philocheras trispinosus (Decapoda) in Port Erin Bay, Isle of Man. Journal of Crustacean Biology 19, 467–476.CrossRefGoogle Scholar
Petersen, S. and Anger, K. (1997) Chemical and physiological changes during embryonic development of the spider crab, Hyas araneus L. (Decapoda: Majidae). Comparative Biochemistry and Physiology—Part B 117, 299–306.CrossRefGoogle Scholar
Rainuzzo, J.R., Reitan, K.I. and Olsen, Y. (1997) The significance of lipids at early stages of marine fish: a review. Aquaculture 155, 103–116.CrossRefGoogle Scholar
Rosa, R. and Nunes, M.L. (2003) Tissue biochemical composition in relation to the reproductive cycle of deep-sea decapod Aristeus antennatus on the south Portuguese coast. Journal of the Marine Biological Association of the United Kingdom 83, 963–970.Google Scholar
Rosa, R., Calado, R., Narciso, L. and Nunes, M.L. (2007) Embryogenesis of decapod crustaceans with different life history traits, feeding ecologies and habitats: a fatty acid approach. Marine Biology 151, 935–947.CrossRefGoogle Scholar
Scott, C., Kwasniewski, S., Falk-Petersen, S. and Sargent, J.R. (2002) Species differences, origins and functions of fatty acids in the wax esters and phospholipids of Calanus hyperboreus, C. glacialis and C. finmarchicus from Artic waters. Marine Ecology Progress Series 235, 127–134.CrossRefGoogle Scholar
Sørensen, T.F., Drillet, G., Engell-Sørensen, K., Hansen, B.W. and Ramløv, H. (2007) Production and biochemical composition of eggs from neritic calanoid copepods reared in large outdoor tanks (Limfjord, Denmark). Aquaculture 263, 84–96.Google Scholar
Sorgeloos, P., Dhert, P. and Candreva, P. (2001) Use of the brine shrimp, Artemia spp., in marine fish larviculture. Aquaculture 200, 147–159.CrossRefGoogle Scholar
Staton, J.L. and Sulkin, S.D. (1991) Nutritional requirements and starvation resistance in larvae of the brachyuran crabs Sesarma cinereum (Bosc) and S. reticulatum (Say). Journal of Experimental Marine Biology and Ecology 152, 271–284.Google Scholar
Urcera, M.J., Anaiz, R., Rua, N. and Coo, A. (1993) Cultivo de la centolla Maja squinado: Influenza de la dieta en el desarrollo larvario. Actas del IV Congreso Nacional de Acuicultura, Isla de Arosa, Spain, 269–274.Google Scholar
Volkman, J.K., Barrett, S.M., Blackburn, S.I., Mansour, M.P., Sikes, E.L. and Gelin, F. (1998) Microalgal biomarkers: a review of recent research developments. Organic Geochemistry 29, 1163–1179.CrossRefGoogle Scholar
Wear, R.G. (1974) Incubation in British decapod crustaceans, and the effect of temperature on the rate of success of embryonic development. Journal of the Marine Biological Association of the United Kingdom 54, 745–762.CrossRefGoogle Scholar
Wehrtmann, I.S. and Graeve, M. (1998) Lipid composition and utilization in developing eggs of two tropical marine caridean shrimps (Decapoda: Caridea: Alpheidae, Palaemonidae). Comparative Biochemistry and Physiology—Part B 121, 457–463.Google Scholar
Wehrtmann, I.S. and Kattner, G. (1998) Changes in volume, biomass, and fatty acids of developing eggs in Nauticaris magellanica (Decapoda: Caridea): a latitudinal comparison. Journal of Crustacean Biology 18, 413–422.Google Scholar