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Ecdysteroids in female shore crabs Carcinus maenas during the moulting cycle and oocyte development

Published online by Cambridge University Press:  14 May 2008

Bjarne Styrishave ,
Torben Lund  and
Ole Andersen
Bjarne Styrishave*
Affiliation:
Department of Science, Systems and Models, University of Roskilde, PO Box 260, 4000 Roskilde, Denmark
Torben Lund
Affiliation:
Department of Science, Systems and Models, University of Roskilde, PO Box 260, 4000 Roskilde, Denmark
Ole Andersen
Affiliation:
Department of Science, Systems and Models, University of Roskilde, PO Box 260, 4000 Roskilde, Denmark
*
Correspondence should be addressed to: Bjarne Styrishave Department of Science, Systems and ModelsUniversity of RoskildePO Box 260, 4000 RoskildeDenmark email: [email protected]
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Abstract

The ecdysteroids ecdysone (E), 20-hydroxyecdysone (20E) and ponasterone A (PoA) were measured in the haemolymph, hepatopancreas and oocytes of female shore crabs Carcinus maenas during the moulting cycle and during oocyte maturation using HPLC-MS. In the haemolymph, ecdysteroid titres varied over the moulting cycle with high levels during premoult and low levels during postmoult and intermoult, however, no significant change in haemolymph ecdysteroid titres was observed in relation to oocyte development. In the hepatopancreas, PoA levels were high during premoult but low during postmoult and intermoult. This is in contrast to E and 20E where levels remained high from early intermoult (C1) until late premoult (D3) and only decreased during postmoult. In the oocytes, ecdysteroid levels were low during postmoult and for 20E and PoA also during late premoult D2 and D3. In contrast, all three ecdysteroids were observed to increase in the oocytes during oocyte development, in particular E and PoA. The present study demonstrates that changes in haemolymph ecdysteroid titres relate to changes in moulting status and not to changes in oocyte development. Also, the study indicates that the hepatopancreas is involved in the metabolism of ecdysteroids related to the moulting cycle but may also be involved in ecdysteroid metabolism during oocyte development. Furthermore, the pronounced increase in oocyte ecdysteroids during oocyte development during periods where haemolymph ecdysteroids titre is low, indicates that the oocytes are capable of de novo synthesis of the three ecdysteroids investigated.

Keywords

Carcinus maenasecdysonegrowth20-hydroxyecdysoneponasterone Areproductionshore crab
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 88 , Issue 3 , May 2008 , pp. 575 - 581
Copyright
Copyright © Marine Biological Association of the United Kingdom 2008

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