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The bivalve mollusc Mactra corallina: genetic evidence of existing sibling species

Published online by Cambridge University Press:  12 February 2010

I. Guarniero ,
F. Plazzi ,
A. Bonfitto ,
A. Rinaldi ,
M. Trentini  and
M. Passamonti
I. Guarniero*
Affiliation:
Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
F. Plazzi
Affiliation:
Department of Evolutionary and Experimental Biology, Faculty of Mathematical, Physical and Natural Sciences, University of Bologna, Via Selmi 3, 40126 Bologna (BO), Italy
A. Bonfitto
Affiliation:
Department of Evolutionary and Experimental Biology, Faculty of Mathematical, Physical and Natural Sciences, University of Bologna, Via Selmi 3, 40126 Bologna (BO), Italy
A. Rinaldi
Affiliation:
Oceanographic Structure Daphne, ARPA Emilia Romagna, Viale Vespucci, 2–47042 Cesenatico (FC), Italy
M. Trentini
Affiliation:
Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
M. Passamonti
Affiliation:
Department of Evolutionary and Experimental Biology, Faculty of Mathematical, Physical and Natural Sciences, University of Bologna, Via Selmi 3, 40126 Bologna (BO), Italy
*
Correspondence should be addressed to: I. Guarniero, Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy email: [email protected]
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Abstract

The rayed trough-shell Mactra corallina Linnaeus 1758 is a surf clam that inhabits the Atlantic Ocean, Black Sea and Mediterranean Sea and represents a commercially important bivalve. This species is present with two different and well-defined sympatric morphotypes, which differ mainly for the colour of the shell (white in the corallina morph, and brown-banded in the lignaria morph). The aim of this work is to resolve the confused and contradictory systematics of the bivalves belonging to M. corallina putative species by analysing molecular and morphological features. Fifteen specimens of M. corallina corallina (white variant) and 19 specimens of M. corallina lignaria (brown variant) were collected in the North Adriatic Sea and analysed by four molecular markers (12S, 16S, 18S and COI genes, partial sequences). Genetic analyses clearly support the presence of two different species, which were previously ascribed to M. corallina. In addition, 35 specimens identified on a morphological basis as M. c. corallina and 28 specimens identified as M. c. lignaria collected in the same area were used for a morphometric analysis. A positive correlation was found between the maximum width of shell (W), antero-posterior length and between W and the height of specimens from umbo to ventral margin, thus adding to molecular data.

Keywords

genetic diversitymolecular taxonomybivalvesMactra
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Issue 3 , May 2010 , pp. 633 - 644
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

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References

REFERENCES

Adamkewicz, S.L., Harasewych, M.G., Blake, J., Saudek, D. and Bult, C. (1997) A molecular phylogeny of the bivalve mollusks. Molecular Biology and Evolution 14, 619–629.CrossRefGoogle ScholarPubMed
Backeljau, T., Bouchet, P., Gofas, S. and de Bruyn, L. (1994) Genetic variation, systematics and distribution of the venerid clam Chamelea gallina. Journal of the Marine Biological Association of the United Kingdom 74, 211–223.CrossRefGoogle Scholar
Chiarelli, S. (1999) Nuovo Catalogo delle Conchiglie Marine del Mediterraneo. Società Italiana di Malacologia, http://www.aicon.com/sim/index.html.Google Scholar
Conroy, A.M., Smith, P.J., Michael, K.P. and Stotter, D.R. (1993) Identification and recruitment patterns of juvenile surf clams, Mactra discors and M. murchisoni from central New Zealand. New Zealand Journal of Marine and Freshwater Research 27, 279–285.CrossRefGoogle Scholar
D'Angelo, G. and Gargiulo, S. (1987) Mactridae. In Fabbri, (ed.) Guida alle conchiglie mediterranee. Milan: Gruppo editoriale Fabbri spa, pp. 192–193.Google Scholar
Excoffier, L., Smouse, P. and Quattro, J. (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131, 479–491.CrossRefGoogle ScholarPubMed
Excoffier, L., Laval, G. and Schneider, S. (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1, 47–50.Google Scholar
Fischer, W., Schneider, M. and Bauchot, M.L. (1987) Fiches FAO d'identification des espèces pour les besoins de la pêche. Mêditerranêe et Mêr Noire. Zone de pêche 37. Végétaux et invertébrés. Rome: FAO Publication.Google Scholar
Folmer, O., Black, M., Hoeh, W.R., Lutz, R. and Vrijenhoek, R.C. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.Google ScholarPubMed
Garrido-Ramos, M.S., Stewart, D.T, Sutherland, B.W. and Zouros, E. (1998) The distribution of male-transmitted and female-transmitted mitochondrial DNA types in somatic tissues of blue mussels: implications for the operation of doubly uniparental inheritance of mitochondrial DNA. Genome 41, 818–824.CrossRefGoogle Scholar
Hou, L., Lü, H., Zou, X., Bi, X., Yan, D. and He, C. (2006) Genetic characterizations of Mactra veneriformis (Bivalve) along the Chinese coast using ISSR-PCR markers. Aquaculture 261, 865–871.CrossRefGoogle Scholar
Huelsenbeck, J.P. and Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17, 754–755.CrossRefGoogle Scholar
Ihaka, R. and Gentleman, R. (1996). R: A language for data analysis and graphics. Journal of Computational and Graphical Statistics 5, 299–314.Google Scholar
Katoh, K. and Toh, H. (2008) Improved accuracy of multiple ncRNA alignment by incorporating structural information into a MAFFT-based framework. BMC Bioinformatics 9, 212.CrossRefGoogle ScholarPubMed
Katoh, K., Kuma, K.I., Toh, H. and Miyata, T. (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Research 33, 511–518.CrossRefGoogle ScholarPubMed
Katoh, K., Misawa, K., Kuma, K.I. and Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30, 3059–3066.CrossRefGoogle ScholarPubMed
Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotides sequences. Journal of Molecular Evolution 16, 111–120.CrossRefGoogle ScholarPubMed
Kimura, M. (1981) Estimation of evolutionary distances between homologous nucleotide sequences. Proceedings of the National Academy of Sciences of the USA 78, 454–458.CrossRefGoogle ScholarPubMed
Livi, S., Cordisco, C., Damiani, C., Romanelli, M. and Crosetti, D. (2006) Identification of bivalve species at an early developmental stage through PCR–SSCP and sequence analysis of partial 18S rDNA. Marine Biology 149: 1149–1161.CrossRefGoogle Scholar
Øines, Ø. and Heuch, P.A. (2005) Identification of sea louse species of the genus Caligus using mtDNA. Journal of the Marine Biological Association of the United Kingdom 85, 73–79.CrossRefGoogle Scholar
Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. and Grabowski, G. (1996) The simple fool's guide to PCR. Hawaii, USA: Kewalo Marine Laboratory and University of Hawii.Google Scholar
Parenzan, P. (1976) Carta d'identità delle conchiglie del Mediterraneo. Volume II. Bivalvi. Taranto, Italy: Bios Taras Editrice, 546 pp.Google Scholar
Passamaneck, Y.J., Schandler, C. and Halanych, K.M. (2004). Investigation of mulluscan phylogeny using large-subunit and small-subunit nuclear rRNA sequences. Molecular Phylogenetic Evolution 32, 25–38.CrossRefGoogle Scholar
Passamonti, M. and Ghiselli, F. (2009) Doubly uniparental inheritance: two mitochondrial genomes, one precious model for organelle DNA inheritance and evolution. DNA and Cell Biology 28, 1–10.CrossRefGoogle ScholarPubMed
Posada, D. and Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.CrossRefGoogle ScholarPubMed
R development Core Team (2009) R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. ISBN 3-900051-07-0, URL http://www.R-project.org.Google Scholar
Riedl, R. (1991) Mactridae. In Muzzio, F. (ed.) Fauna e flora del Mediterraneo. Dalle alghe ai mammiferi: una guida sistematica alle specie che vivono nel Mar Mediterraneo. Trento: Legoprint srl, pp. 342–343.Google Scholar
Ronquist, F. and Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.CrossRefGoogle ScholarPubMed
Simmons, M.P. and Ochoterena, H. (2000) Gaps as characters in sequence-based phylogenetic analyses. Systematic Biology 49, 369–381.CrossRefGoogle ScholarPubMed
Simon, C., Buckley, T.R., Frati, F., Stewart, J.B. and Beckenbach, A.T. (2006) Incorporating molecular evolution into phylogenetic analysis, and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA. Annual Review of Ecology and Systematics 37, 545–579.CrossRefGoogle Scholar
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.CrossRefGoogle ScholarPubMed
Taylor, J.D., Williams, S.T., Glover, E.A. and Dyal, P. (2007) A molecular phylogeny of heterodont bivalves (Mollusca: Bivalvia: Heterodonta): new analyses of 18S and 28S rRNA genes. Zoologica Scripta 36, 587–606.CrossRefGoogle Scholar
Young, N.D. and Healy, J. (2003) GapCoder automates the use of indel characters in phylogenetic analysis. BMC Bioinformatics 4, 6.CrossRefGoogle ScholarPubMed
Zuccon, A. and Zuccon, D. (2006) MrEnt v2.0. Program distributed by the authors, Department of Vertebrate Zoology & Molecular Systematics Laboratory, Swedish Museum of Natural History, Stockholm.Google Scholar