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Aerobic and anaerobic enzymatic activities of Calyptogena gallardoi (Vesicomyidae): a clam associated with methane cold seeps off Chile

Published online by Cambridge University Press:  25 July 2008

R.R. González ,
R.A. Quiñones ,
E. Quiroga  and
J. Sellanes
R.R. González*
Affiliation:
Centro de Investigación Oceanográfica en el Pacífico Sur-Oriental (COPAS), Universidad de Concepción, Casilla 160C, Concepción, Chile Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla 160C, Concepción, Chile
R.A. Quiñones
Affiliation:
Centro de Investigación Oceanográfica en el Pacífico Sur-Oriental (COPAS), Universidad de Concepción, Casilla 160C, Concepción, Chile Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla 160C, Concepción, Chile
E. Quiroga
Affiliation:
Centro de Investigación en Ecosistemas de la Patagonia (CIEP), Bilbao 449, Coyhaique, Chile
J. Sellanes
Affiliation:
Centro de Investigación Oceanográfica en el Pacífico Sur-Oriental (COPAS), Universidad de Concepción, Casilla 160C, Concepción, Chile Departamento de Biología Marina, Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile
*
Correspondence should be addressed to: Centro de Investigación Oceanográfica en el Pacífico Sur-Oriental (COPAS) Universidad de Concepción Casilla 160C, Concepción, Chile email: [email protected]
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Abstract

Calyptogena gallardoi is a recently described species of vesicomyid clam associated to the extensive gas-hydrate field reported for the Chilean margin along 35°S to 45°S. Enzymatic analysis in foot, gill and abductor muscle tissues of C. gallardoi collected in central-south Chile (~36°21′S 73°44′W), show high activities for malate dehydrogenase (MDH), strombine dehydrogenase (STRDH) and alanopine dehydrogenase (ALPDH) and a low activity of citrate synthase (CS) and ETS (electron transport system). Positive significant correlations (log–log scale) were found between enzymatic activities involved in anaerobic metabolism (MDH versus LDH and ALPDH versus STRDH), as well as between CS and opines dehydrogenases. The ratio MDH/LDH or any other opine dehydrogenase assayed was >> 1 in all tissues analysed. These results indicate that C. gallardoi is highly adapted to the harsh anaerobic conditions of marine chemosynthesis-based communities inhabiting a reduced environment.

Keywords

aerobicanaerobicenzymesCalyptogena gallardoimethane cold seepsChile
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 88 , Issue 5 , August 2008 , pp. 983 - 986
Copyright
Copyright © Marine Biological Association of the United Kingdom 2008

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