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Metal-Containing Blood Cells of Oysters: Ultrastructure, Histochemistry and X-Ray Microanalysis

Published online by Cambridge University Press:  11 May 2009

Brian J. S. Pirie ,
Stephen G. George ,
David G. Lytton  and
John D. Thomson
Brian J. S. Pirie
Affiliation:
N.E.R.C. Institute of Marine Biochemistry, St Fittick's Road, Aberdeen
Stephen G. George
Affiliation:
N.E.R.C. Institute of Marine Biochemistry, St Fittick's Road, Aberdeen
David G. Lytton
Affiliation:
AB 3RA Department of Pathology, University of Tasmania, Hobart, Tasmania 7001
John D. Thomson
Affiliation:
Australia Tasmanian Fisheries Development Authority, 23 Old Wharf, Hobart, Tasmania 7000, Austlia
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Extract

The ultrastructure, histochemistry and X-ray microanalysis of the metal-containing blood cells in three species of oyster, Ostrea edulis, Ostrea angasi and Crassostrea gigas are described. Granular cells containing both copper (up to 400 mM) and zinc (up to 1 M) are present in all three species. In addition,Ostrea edulis has two other granular amoebocyte types which specifically accumulate either copper (400 mM) or zinc (12 M). In all types of amoebocyte, the metals are stored in membrane-limited vesicles. The ultrastructure and histochemical staining characteristics of the vesicular contents vary according to metal composition. Zinc-containing granules appear basophilic after sulphide treatment and display homogeneous electron density; copper/zinc-containing granules are less dense and show varying degrees of crystallinity; copper-containing granules appear crystalline (however, any acidophilic or basophilic reaction is masked by the dark brown precipitate of copper sulphide).

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 64 , Issue 1 , February 1984 , pp. 115 - 123
Copyright
Copyright © Marine Biological Association of the United Kingdom 1984

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