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The lysidyl aminoacyl transfer RNA synthetase intron, a new marker for demosponge phylogeographics – case study on Neopetrosia

Published online by Cambridge University Press:  12 November 2015

Edwin Setiawan ,
Nicole J. De Voogd ,
John N.A. Hooper ,
Gert Wörheide  and
Dirk Erpenbeck
Edwin Setiawan
Affiliation:
Department of Earth and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-University Munich, Munich, Germany Zoology Lab, Biology Department, Mathematic and Natural Science Faculty, Sepuluh November Institute of Technology, Surabaya, Indonesia Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, the Netherlands
Nicole J. De Voogd
Affiliation:
Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, the Netherlands
John N.A. Hooper
Affiliation:
Biodiversity Program, Queensland Museum, South Brisbane, Australia Eskitis Institute for Drug Discovery, Griffith University, Nathan, Queensland 4111, Australia
Gert Wörheide
Affiliation:
Department of Earth and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-University Munich, Munich, Germany GeoBio-Center LMU Ludwig-Maximilians-University Munich, Richard-Wagner-Str. 10, 80333 Munich, Germany SNSB – Bayerische Staatssammlung für Paläontologie und Geologie, Munich 80333, Germany
Dirk Erpenbeck*
Affiliation:
Department of Earth and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-University Munich, Munich, Germany GeoBio-Center LMU Ludwig-Maximilians-University Munich, Richard-Wagner-Str. 10, 80333 Munich, Germany
*
Correspondence should be addressed to:D. Erpenbeck, Department of Earth and Environmental Sciences & GeoBio-Center LMU, Ludwig-Maximilians-University Munich, Richard-Wagner-Str. 10, 80333 Munich, Germany Email: [email protected]
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Abstract

Suitable genetic markers for population studies in sponges are necessary to further our understanding of biodiversity and dispersal patterns, and contribute to conservation efforts. Due to the slow mitochondrial substitution rates in demosponges, nuclear introns are among the preferable markers for phylogeographic studies, but so far only the second intron of the ATP synthetase beta subunit-gene (ATPSβ) has been successfully established. In the present study, we analyse the intron of the Lysidyl Aminoacyl Transfer RNA Synthetase (LTRS), another potential marker to study demosponge intraspecific relationships, on samples of Neopetrosia chaliniformis from various locations in the Indo-Pacific and compare its variation with a mitochondrial marker (CO2). LTRS recovers several reciprocal monophyletic groups among the Indo-Pacific N. chaliniformis and provides a potential alternative to ATPSβ.

Keywords

PoriferaspongesNeopetrosiamarkernuclear DNAintronlysidyl aminoacyl transfer RNA synthetaseLTRS
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 96 , Issue 2: Proceedings of the 9th World Sponge Conference held in Fremantle Australia in 2013: New Frontiers in Sponge Science , March 2016 , pp. 333 - 339
Copyright
Copyright © Marine Biological Association of the United Kingdom 2015 

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