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Fatty acids from controlled feeding as dietary markers of juvenile Chinese horseshoe crab, Tachypleus tridentatus

Published online by Cambridge University Press:  15 April 2018

Billy K.Y. Kwan ,
Menghong Hu ,
Youji Wang ,
Siu Gin Cheung  and
Paul K.S. Shin
Billy K.Y. Kwan
Affiliation:
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou, China College of Ocean, Qinzhou University, Qinzhou, China Department of Biology and Chemistry, City University of Hong Kong, Hong Kong
Menghong Hu
Affiliation:
Department of Biology and Chemistry, City University of Hong Kong, Hong Kong National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, China
Youji Wang
Affiliation:
Department of Biology and Chemistry, City University of Hong Kong, Hong Kong National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, China
Siu Gin Cheung
Affiliation:
Department of Biology and Chemistry, City University of Hong Kong, Hong Kong State Key Laboratory in Marine Pollution, City University of Hong Kong, Hong Kong
Paul K.S. Shin*
Affiliation:
Department of Biology and Chemistry, City University of Hong Kong, Hong Kong State Key Laboratory in Marine Pollution, City University of Hong Kong, Hong Kong
*
Correspondence should be addressed to: P.K.S. Shin, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong email: [email protected]
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Abstract

The potential use of fatty acid (FA) profile as a dietary tracer in juvenile Chinese horseshoe crab Tachypleus tridentatus was assessed in a feeding experiment. Selected feed was provided to the juveniles at various growth stages according to their food sources in the wild: larval and adult brine shrimp Artemia salina for the first six months, followed by a mixture of short-neck clam Ruditapes philippinarum and greasyback shrimp Metapenaeus ensis for a further six months. Juvenile horseshoe crab haemolymph and tissues of book gill, opisthosomal spine, chelicera and swimming leg were sampled after the feeding experiment. The FA profiles revealed that the juvenile haemolymph and various tissues had a total of 23–24 FA components. The major FA components consisted of saturated (palmitic and stearic) at 22–34%, monosaturated (oleic) at 7–34% and polyunsaturated (eicosapentaenoic and arachidonic) FAs at 9–27%. According to principal component analysis, the FA signatures of juvenile haemolymph were greatly influenced by the shift of food sources from brine shrimp to clam and shrimp meat, whereas the FA profiles of different tissues were more resistant to the nutrient alteration. The present findings confirmed that the FA profile of both juvenile haemolymph and different tissues reflected the FA composition of recent and previous diets, respectively; and thus may be useful in indicating the foraging information of juvenile horseshoe crabs in the field.

Keywords

Nutritious dietfeeding nichehorseshoe crab instarlaboratory assaygas chromatography analysisfatty acid species
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 99 , Issue 2 , March 2019 , pp. 421 - 428
Copyright
Copyright © Marine Biological Association of the United Kingdom 2018 

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Footnotes

*

B.K.Y. Kwan and M. Hu are co-first authors.

References

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