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Exploring cryptic diversity in publicly available strains of the model diatom Thalassiosira pseudonana (Bacillariophyceae)

Published online by Cambridge University Press:  17 April 2015

Cecilia Rad-Menéndez ,
Michele Stanley ,
David H. Green ,
Eileen J. Cox  and
John G. Day
Cecilia Rad-Menéndez*
Affiliation:
Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, UK Microbial & Molecular Biology Department, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, UK
Michele Stanley
Affiliation:
Microbial & Molecular Biology Department, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, UK
David H. Green
Affiliation:
Microbial & Molecular Biology Department, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, UK
Eileen J. Cox
Affiliation:
Department of Botany, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
John G. Day
Affiliation:
Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, UK Microbial & Molecular Biology Department, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, UK
*
Correspondence should be addressed to: C. Rad-Menéndez, Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, UK email: [email protected]
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Abstract

The model diatom Thalassiosira pseudonana is believed to be a single species with a global distribution, but it has not been confirmed previously whether isolates from different environmental and geographic origins are genotypically and phenotypically identical. In the present study, a polyphasic approach was employed to characterize nine clonal isolates, plus an additional replicate of one of the isolates, of the diatom T. pseudonana from culture collections to investigate whether there was any cryptic speciation in the publicly available strains of this species. Morphological analysis using scanning electron microscopy concluded that the strains were indistinguishable. Furthermore, conventional DNA barcoding genes (SSU rDNA, ITS1 and ITS2 rDNA and rbcL), revealed no nucleotide variation among the strains tested. On employing a whole genome fingerprinting technique, Amplified Fragment Length Polymorphism (AFLP), three clusters were revealed, although the level of variation between the clusters was surprisingly low. These findings indicate a low level of diversity among these cultured T. pseudonana strains, despite their wide spatial and temporal distribution and the salinity range of their original habitats. Based on the limited number of available strains, this suggests that T. pseudonana is a highly conserved diatom that nevertheless has an ability to tolerate wide ranges of salinity and populate varied geographic locations.

Keywords

Amplified Fragment Length Polymorphism (AFLP)DNA barcodingfingerprintingThalassiosira pseudonanaCyclotella nana
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 95 , Issue 6 , September 2015 , pp. 1081 - 1090
Copyright
Copyright © Marine Biological Association of the United Kingdom 2015 

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