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Effects of polyploidy on response of Dunaliella salina to salinity

Published online by Cambridge University Press:  04 February 2019

Fatemeh Soltani Nezhad  and
Hakimeh Mansouri
Fatemeh Soltani Nezhad
Affiliation:
Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
Hakimeh Mansouri*
Affiliation:
Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
*
Author for correspondence: Hakimeh Mansouri, E-mail: [email protected]
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Abstract

In this study, polyploidy level was determined by flow cytometry analysis. The effect of polyploidy by colchicine treatment was examined on the growth parameters, malondealdehyde (MDA), as well as activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in response to different levels of salinity in Dunaliella salina. The results of algal growth indicated that 3 M NaCl was the optimal concentration of salt, since the highest enhancement in fresh and dry weight, chlorophyll and carotenoids, soluble sugar, glycerol, protein and starch content was observed in comparison to other concentrations. The amount of these metabolites declined in the concentrations under optimum salinity. The least and highest amounts of MDA were observed at 1 and 4 M NaCl respectively. Polyploidy in optimum concentration of salt, caused further increment of the above growth parameters. In relation to this, in most cases, treatment of 0.1% colchicine was most effective. The beneficial effects of polyploidy in non-optimal conditions were also found in some parameters such as biomass, chlorophyll, carotenoids, proteins and starch. Furthermore, the activity of antioxidant enzymes CAT, SOD and POD showed a positive significant correlation with salt stress and these were maximized at 4 M NaCl. Polyploidy (especially colchicine 0.1%) affected activity of these antioxidant enzymes in some concentrations of salt. Overall, our results suggest that the microalgae has significantly different responses to salt stress based on ploidy levels.

Keywords

Antioxidant enzymescarotenoidDunaliella salinaglycerolpolyploidy
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 99 , Issue 5 , August 2019 , pp. 1041 - 1047
Copyright
Copyright © Marine Biological Association of the United Kingdom 2019 

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