Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-25T13:27:38.431Z Has data issue: false hasContentIssue false
  • English
  • Français

Duplication of the ribosomal gene cluster in the marine polychaete Platynereis dumerilii correlates with ITS polymorphism

Published online by Cambridge University Press:  04 April 2007

Jerome H.L. Hui ,
Natalia Kortchagina ,
Detlev Arendt ,
Guillaume Balavoine  and
David E.K. Ferrier
Jerome H.L. Hui
Affiliation:
Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road, Oxford, OX1 3PS, UK
Natalia Kortchagina
Affiliation:
Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road, Oxford, OX1 3PS, UK
Detlev Arendt
Affiliation:
Developmental Unit, European Molecular Biological Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany
Guillaume Balavoine
Affiliation:
Centre de Genetique Moleculaire du CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France
David E.K. Ferrier
Affiliation:
Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road, Oxford, OX1 3PS, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

Platynereis dumerilii is an emerging model system for both comparative developmental biology and ecotoxicology. As such it would be extremely valuable to be able to map the location of genes and markers in its genome, in order to understand how the genome changes or has changed, either due to chemical and environmental insults or during evolution. Here we report the adaptation of the technique of chromosomal fluorescence in situ hybridization (FISH) to P. dumerilii for the localization of single-copy genes. Also the duplicated ribosomal gene cluster of this annelid is more reliably revealed with FISH than with the previous technique of silver staining. This duplicated ribosomal cluster correlates with polymorphisms in the internal transcribed spacer (ITS) regions, which has implications for the use of these regions in phylogenetic or population biology work.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 87 , Issue 2 , April 2007 , pp. 443 - 449
Copyright
2007 Marine Biological Association of the United Kingdom

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)