Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-18T16:01:31.907Z Has data issue: false hasContentIssue false
  • English
  • Français

Distribution pattern of GFP (green fluorescent protein) in a bivalve-inhabiting hydrozoan, Eutima japonica (Leptomedusae: Eirenidae)

Published online by Cambridge University Press:  14 July 2010

Shin Kubota ,
Eriko Nomaru ,
Hiroko Uchida  and
Akio Murakami
Shin Kubota*
Affiliation:
Seto Marine Biological Laboratory, Field Science Education and Research Center, Kyoto University, Shirahama, Nishimuro, Wakayama 649-2211, Japan
Eriko Nomaru
Affiliation:
Kobe University Research Center for Inland Seas, Iwaya 2746, Awaji, Hyogo, 656-401, Japan
Hiroko Uchida
Affiliation:
Kobe University Research Center for Inland Seas, Iwaya 2746, Awaji, Hyogo, 656-401, Japan
Akio Murakami
Affiliation:
Kobe University Research Center for Inland Seas, Iwaya 2746, Awaji, Hyogo, 656-401, Japan
*
Correspondence should be addressed to: S. Kubota, Seto Marine Biological Laboratory, Field Science Education and Research Center, Kyoto University, Shirahama, Nishimuro Wakayama 649-2211Japan email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Bright green auto-fluorescence was observed in the umbrellar margin, umbrellar marginal warts, tentacular bulbs, tentacles, and manubrium of laboratory-reared immature (1–14 days old) medusae of Eutima japonica from Japan and China. In vivo microscopic fluorescence spectra showed that the green fluorescence was similar to that of green fluorescent protein (GFP) found in Aequorea victoria, although the maximum emission wavelength (503 nm) was slightly bluer. No fluorescence was detected in the cirri, statocysts, radial canals, velum, or subumbrella of the medusae. The fluorescence distribution pattern in E. japonica more closely resembles that of Eugymnanthea inquilina from the Mediterranean Sea than that of Japanese Eugymnanthea japonica, which is the derived species of E. japonica. This suggests that the common fluorescence pattern is convergently evolved in the former two species, perhaps owing to the as yet unclarified physiological and/or ecological function of GFP and/or GFP-like proteins.

Keywords

fluorescence pattern and spectragreen fluorescent protein (GFP)hydromedusaEutimaEugymnantheasystematics
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Issue 7 , November 2010 , pp. 1371 - 1374
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Belogurova, N.V., Kudryasheva, N.S., Alieva, R.R. and Sizykh, A.G. (2008) Spectral components of bioluminescence of aequorin and obelin. Journal of Photochemistry and Photobiology B: Biology 92, 117–122.CrossRefGoogle ScholarPubMed
Deheyn, D., Kubokawa, K., McCarthy, J.K., Murakami, A., Porrachia, M., Rouse, G.W. and Holland, N.D. (2007) Endogenous green fluorescent protein (GFP) in amphioxus. Biological Bulletin. Marine Biological Laboratory, Woods Hole 213, 95–100.CrossRefGoogle ScholarPubMed
Govindarajan, A.F., Piraino, S., Gravili, C. and Kubota, S. (2005) Species identification of bivalve-inhabiting marine hydrozoans of the genus Eugymnanthea. Invertebrate Biology 124, 1–10.CrossRefGoogle Scholar
Haddock, S.H.D. and Case, J.F. (1999) Bioluminescence spectra of shallow and deep-sea gelatinous zooplankton: ctenophores, medusae and siphonophores. Marine Biology, Berlin 133, 571–582.CrossRefGoogle Scholar
Kubota, S. (1992) Four bivalve-inhabiting hydrozoans in Japan differing in range and host preference. Scientia Marina 56, 149–159.Google Scholar
Kubota, S. (2000) Parallel, paedomorphic evolutionary processes of the bivalve-inhabiting hydrozoans (Leptomedusae, Eirenidae) deduced from the morphology, life cycle and biogeography, with special reference to taxonomic treatment of Eugymnanthea. Scientia Marina 64, Supplement 1, 241–247.CrossRefGoogle Scholar
Kubota, S. (2004) Some new and reconfirmed biological observations in two species of Eugymnanthea (Hydrozoa, Leptomedusae, Eirenidae) associated with bivalves. Biogeography 6, 1–5.Google Scholar
Kubota, S. (2008) Life cycle of a bivalve-inhabiting hydrozoan, Eutima japonica (Hydrozoa, Leptomedusae) in Tsingtao, China and determination of its form by culture. Bulletin of the Biogeographical Society of Japan 63, 145–149. [In Japanese with English summary.]Google Scholar
Kubota, S., Pagliara, P. and Gravili, C. (2008) Fluorescence distribution pattern allows to distinguish two species of Eugymnanthea (Leptomedusae: Eirenidae). Journal of the Marine Biological Association of the United Kingdom 88, 1743–1746.CrossRefGoogle Scholar
Murakami, A., Miyashita, H., Iseki, M., Adachi, K. and Mimuro, M. (2004) Chlorophyll d in an epiphytic cyanobacterium on red algae. Science 303, 1633.CrossRefGoogle Scholar
Shimomura, O. (2006) Bioluminescence: chemical principles and methods. New Jersey: World Scientific Publishing Company, 470 pp.CrossRefGoogle Scholar
Shimomura, O., Johnson, F.H. and Saiga, Y. (1962) Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. Journal of Cellular and Comparative Physiology 59, 223–239.CrossRefGoogle ScholarPubMed
Ward, W.W. (1998) Biochemical and physical properties of green fluorescent protein. In Green Fluorescent Protein: Properties, Applications, and Protocols. In Chalfie, M. and Kain, S. (eds) Methods in cell biology. New York: Wiley-Liss, pp. 45–75.Google Scholar
Yanushevich, Y.G., Shagin, D.A., Fradkov, A.F., Shakhbazov, K.S., Barsova, E.V., Gurskaya, N.G., Labas, Y.A., Matz, A.V., Lukyanov, K.A. and Lukyanov, S.A. (2005) Spectral diversity among members of the green fluorescent protein family in hydroid jellyfish (Cnidaria, Hydrozoa). Russian Journal of Bioorganic Chemistry 31, 43–47.CrossRefGoogle Scholar