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A versatile method for generating single DNA molecule patterns: Through the combination of directed capillary assembly and (micro/nano) contact printing

Published online by Cambridge University Press:  24 January 2011

Aline Cerf ,
Xavier Dollat ,
Jérôme Chalmeau ,
Angélique Coutable  and
Christophe Vieu
Aline Cerf*
Affiliation:
CNRS, LAAS, F-31077 Toulouse, France; and Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077 Toulouse, France
Xavier Dollat
Affiliation:
CNRS, LAAS, F-31077 Toulouse, France; and Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077 Toulouse, France
Jérôme Chalmeau
Affiliation:
Physics Department, University of Minnesota, Minneapolis, Minnesota 55416; CNRS, LAAS, F-31077 Toulouse, France; and Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077 Toulouse, France
Angélique Coutable
Affiliation:
Université de Toulouse, INSA, UPS, INP, LISBP, Toulouse F-31077, France; and INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, UMR5504, Toulouse F-31400, France
Christophe Vieu
Affiliation:
CNRS, LAAS, F-31077 Toulouse, France; and Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077 Toulouse, France
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

One of the challenges in the development of molecular scale devices is the integration of nano-objects or molecules onto desired locations on a surface. This integration comprises their accurate positioning, their alignment, and the preservation of their functionality. Here, we proved how capillary assembly in combination with soft lithography can be used to perform DNA molecular combing to generate chips of isolated DNA strands for genetic analysis and diagnosis. The assembly of DNA molecules is achieved on a topologically micropatterned polydimethylsiloxane stamp inducing almost simultaneously the trapping and stretching of single molecules. The DNA molecules are then transferred onto aminopropyltriethoxysilane-coated surfaces. In fact, this technique offers the possibility to tightly control the experimental parameters to direct the assembly process. This technique does not induce a selection in size of the objects, therefore it can handle complex solutions of long (tens of kbp) but also shorter (a few thousands of bp) molecules directly in solution to allow the construction of future one-dimensional nanoscale building templates.

Keywords

BiomaterialLithography (deposition)Nanostructure
Type
Reviews
Information
Journal of Materials Research , Volume 26 , Issue 2: Focus Issue: Self-Assembly and Directed Assembly of Advanced Materials , 28 January 2011 , pp. 336 - 346
Copyright
Copyright © Materials Research Society 2011

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